Studium variabilního úseku onkoproteinu v-Myb
Study of the v-Myb oncoprotein variable region
diplomová práce (OBHÁJENO)
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Trvalý odkaz
http://hdl.handle.net/20.500.11956/95800Identifikátory
SIS: 32564
Kolekce
- Kvalifikační práce [20052]
Autor
Vedoucí práce
Oponent práce
Zadražil, Stanislav
Fakulta / součást
Přírodovědecká fakulta
Obor
Biologie
Katedra / ústav / klinika
Katedra genetiky a mikrobiologie
Datum obhajoby
5. 6. 2007
Nakladatel
Univerzita Karlova, Přírodovědecká fakultaJazyk
Čeština
Známka
Výborně
- 4 - Study of the v-Myb oncoprotein variable region The aim of this thesis was to analyze the significance of the variable region of oncoprotein v-Myb. The v-myb oncogene originated in the chick through a natural recombination of the MAV1 retroviral genome with the c-myb gene, which is an important transcriptional regulator involved in hematopoiesis and other important processes in different types of vertebrate cells. In comparison with the c-myb coding sequence, the retroviral v-myb is truncated on N- and C-terminals and includes 11 point mutations. Due to both these changes and the retroviral promoter which drives its expresion, the v-Myb oncoprotein became the inducer of acute myeloid leukemia in chicks. The variable region of v-Myb lies in between the N-terminal DNA binding and the central transactivating domains. This region is the least evolutionarily conserved part of the protein. No published data are available about its potential function. In this work the biological significance of the variable region was studied by means of specific deletions and sequence swaps. Two deletions (∆PstI and ∆NaeI) and two swaps (chicken v-myb to mouse and Xenopus c-myb) were prepared. Recombinant DNAs were cloned into MAV1-based retroviral vector and transfected into chick embryo fibroblasts (CEFs) in tissue...