Show simple item record

Study of the v-Myb oncoprotein variable region
dc.contributor.advisorDvořák, Michal
dc.creatorStarostová, Michaela
dc.date.accessioned2018-04-18T10:00:56Z
dc.date.available2018-04-18T10:00:56Z
dc.date.issued2007
dc.identifier.urihttp://hdl.handle.net/20.500.11956/95800
dc.description.abstract- 4 - Study of the v-Myb oncoprotein variable region The aim of this thesis was to analyze the significance of the variable region of oncoprotein v-Myb. The v-myb oncogene originated in the chick through a natural recombination of the MAV1 retroviral genome with the c-myb gene, which is an important transcriptional regulator involved in hematopoiesis and other important processes in different types of vertebrate cells. In comparison with the c-myb coding sequence, the retroviral v-myb is truncated on N- and C-terminals and includes 11 point mutations. Due to both these changes and the retroviral promoter which drives its expresion, the v-Myb oncoprotein became the inducer of acute myeloid leukemia in chicks. The variable region of v-Myb lies in between the N-terminal DNA binding and the central transactivating domains. This region is the least evolutionarily conserved part of the protein. No published data are available about its potential function. In this work the biological significance of the variable region was studied by means of specific deletions and sequence swaps. Two deletions (∆PstI and ∆NaeI) and two swaps (chicken v-myb to mouse and Xenopus c-myb) were prepared. Recombinant DNAs were cloned into MAV1-based retroviral vector and transfected into chick embryo fibroblasts (CEFs) in tissue...en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleStudium variabilního úseku onkoproteinu v-Mybcs_CZ
dc.typediplomová prácecs_CZ
dcterms.created2007
dcterms.dateAccepted2007-06-05
dc.description.departmentKatedra genetiky a mikrobiologiecs_CZ
dc.description.departmentDepartment of Genetics and Microbiologyen_US
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId32564
dc.title.translatedStudy of the v-Myb oncoprotein variable regionen_US
dc.contributor.refereeZadražil, Stanislav
dc.identifier.aleph000591272
thesis.degree.nameMgr.
thesis.degree.levelmagisterskécs_CZ
thesis.degree.disciplineBiologyen_US
thesis.degree.disciplineBiologiecs_CZ
thesis.degree.programBiologyen_US
thesis.degree.programBiologiecs_CZ
uk.thesis.typediplomová prácecs_CZ
uk.taxonomy.organization-csPřírodovědecká fakulta::Katedra genetiky a mikrobiologiecs_CZ
uk.taxonomy.organization-enFaculty of Science::Department of Genetics and Microbiologyen_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.csBiologiecs_CZ
uk.degree-discipline.enBiologyen_US
uk.degree-program.csBiologiecs_CZ
uk.degree-program.enBiologyen_US
thesis.grade.csVýborněcs_CZ
thesis.grade.enExcellenten_US
uk.abstract.en- 4 - Study of the v-Myb oncoprotein variable region The aim of this thesis was to analyze the significance of the variable region of oncoprotein v-Myb. The v-myb oncogene originated in the chick through a natural recombination of the MAV1 retroviral genome with the c-myb gene, which is an important transcriptional regulator involved in hematopoiesis and other important processes in different types of vertebrate cells. In comparison with the c-myb coding sequence, the retroviral v-myb is truncated on N- and C-terminals and includes 11 point mutations. Due to both these changes and the retroviral promoter which drives its expresion, the v-Myb oncoprotein became the inducer of acute myeloid leukemia in chicks. The variable region of v-Myb lies in between the N-terminal DNA binding and the central transactivating domains. This region is the least evolutionarily conserved part of the protein. No published data are available about its potential function. In this work the biological significance of the variable region was studied by means of specific deletions and sequence swaps. Two deletions (∆PstI and ∆NaeI) and two swaps (chicken v-myb to mouse and Xenopus c-myb) were prepared. Recombinant DNAs were cloned into MAV1-based retroviral vector and transfected into chick embryo fibroblasts (CEFs) in tissue...en_US
uk.file-availabilityV
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra genetiky a mikrobiologiecs_CZ


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


© 2017 Univerzita Karlova, Ústřední knihovna, Ovocný trh 560/5, 116 36 Praha 1; email: admin-repozitar [at] cuni.cz

Za dodržení všech ustanovení autorského zákona jsou zodpovědné jednotlivé složky Univerzity Karlovy. / Each constituent part of Charles University is responsible for adherence to all provisions of the copyright law.

Upozornění / Notice: Získané informace nemohou být použity k výdělečným účelům nebo vydávány za studijní, vědeckou nebo jinou tvůrčí činnost jiné osoby než autora. / Any retrieved information shall not be used for any commercial purposes or claimed as results of studying, scientific or any other creative activities of any person other than the author.

DSpace software copyright © 2002-2015  DuraSpace
Theme by 
@mire NV