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Mikromanipulace savčích oocytů a embryí - Biologické a epigenetické hledisko
dc.contributor.advisorHozák, Pavel
dc.creatorFulková, Helena
dc.date.accessioned2019-05-03T14:04:27Z
dc.date.available2019-05-03T14:04:27Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/20.500.11956/94331
dc.description.abstractCONCLUSIONS . By antibodies against ďfferent covalent histone modifications and 5-methylcytosine, we have partialty characterised the epigenetic changes taking place during the oocyte mauration and in early mammalian embryogenesis in the mouse and pig, respectively. o We have also characterised thc epigenetic repogramming activities of cytoplasts derived from oocytes at different stages of maturation after somatic cell nuclear transfer. . We have evaluated the epigenetic effec$ of selected procedures that are currently used for embryo production. . Finally, we have developed a new cryopreservation scheme for oocyte nuclear material storage. orrr research is engaged in the development ofnew bíotechniques as well as elucidating and characterising the epigenetic pÍocesses that take place during normal and abnormal embryogenesis. Abnormal embryonic development is for example often observed in somatic cell nuclear transfer embryos. These techniques can also be potentially used not only in human medicine but also for valuable livestock and endangered species preservation Oy e.g. interspecies nuclear transfer). Especially in human meďcine, attention to the ethical issues associated with these techniques must be paid. It is also clear tbat many biological problems still do exist and these should not be...en_US
dc.languageEnglishcs_CZ
dc.language.isoen_US
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleManipulating the mammalian oocyte and embryo - Biological and epigenetic aspectsen_US
dc.typedizertační prácecs_CZ
dcterms.created2008
dcterms.dateAccepted2008-03-26
dc.description.departmentDepartment of Cell Biologyen_US
dc.description.departmentKatedra buněčné biologiecs_CZ
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId112399
dc.title.translatedMikromanipulace savčích oocytů a embryí - Biologické a epigenetické hlediskocs_CZ
dc.contributor.refereeHampl, Aleš
dc.contributor.refereeMotlík, Jan
dc.identifier.aleph002095047
thesis.degree.namePh.D.
thesis.degree.leveldoktorskécs_CZ
thesis.degree.discipline-cs_CZ
thesis.degree.discipline-en_US
thesis.degree.programVývojová biologiecs_CZ
thesis.degree.programDevelopmental Biologyen_US
uk.thesis.typedizertační prácecs_CZ
uk.taxonomy.organization-csPřírodovědecká fakulta::Katedra buněčné biologiecs_CZ
uk.taxonomy.organization-enFaculty of Science::Department of Cell Biologyen_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.cs-cs_CZ
uk.degree-discipline.en-en_US
uk.degree-program.csVývojová biologiecs_CZ
uk.degree-program.enDevelopmental Biologyen_US
thesis.grade.csProspěl/acs_CZ
thesis.grade.enPassen_US
uk.abstract.enCONCLUSIONS . By antibodies against ďfferent covalent histone modifications and 5-methylcytosine, we have partialty characterised the epigenetic changes taking place during the oocyte mauration and in early mammalian embryogenesis in the mouse and pig, respectively. o We have also characterised thc epigenetic repogramming activities of cytoplasts derived from oocytes at different stages of maturation after somatic cell nuclear transfer. . We have evaluated the epigenetic effec$ of selected procedures that are currently used for embryo production. . Finally, we have developed a new cryopreservation scheme for oocyte nuclear material storage. orrr research is engaged in the development ofnew bíotechniques as well as elucidating and characterising the epigenetic pÍocesses that take place during normal and abnormal embryogenesis. Abnormal embryonic development is for example often observed in somatic cell nuclear transfer embryos. These techniques can also be potentially used not only in human medicine but also for valuable livestock and endangered species preservation Oy e.g. interspecies nuclear transfer). Especially in human meďcine, attention to the ethical issues associated with these techniques must be paid. It is also clear tbat many biological problems still do exist and these should not be...en_US
uk.file-availabilityP
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra buněčné biologiecs_CZ
thesis.grade.codeP


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