Stanovení exprese vybraných proteinů apoptotické kaskády v lidském endometriu

Abstract

Apoptosa je programovan a bun e cn a smrt, kter a je vyvol ana nap r. z ava zn ymi mutacemi v DNA nebo bun e cn ym stresem. Hraje d ule zitou roli v udr zov an tk a nov e homeost azy pomoc eliminace star ych, nepot rebn ych nebo po skozen ych bun ek. U castn se j velk e mno zstv molekul, z nich z nejd ule zit ej s jsou z astupci protein u Bcl- 2 rodiny a v ykonn e proteiny apoptosy, kaspasy, zodpov edn e za vlastn proveden apoptosy. Selh an regulace apoptosy m u ze zp usobit radu malignit, v cetn e n adorov e transformace. Lidsk e endometrium je speci ck a tk a n, ve kter e se apoptosa vyskytuje cyklicky. P redlo zen a pr ace se zab yv a studiem exprese protein u Bcl-2, Bax, Bad. Bid, pro-kaspasy-3, kaspasy-3 a PARP v norm aln m, atro ck em, hyperplastick em a kancerozn m (stadia I a II dle FIGO) endometriu. V ysledky t eto studie nazna cuj , ze proteiny Bid a Bad mohou b yt jedn emi z potenci aln ch zlomov ych bod u v procesu n adorov e transformace bu nky, na co z poukazuje i opa cn y vzorec jejich exprese v hyperplastick em a kancerozn m endometriu.

Apoptosis is a process of the programmed cell death in response to severe mutations in DNA or cell stress. Apoptosis plays a key role in tissue maintenance by eliminating senescent and damaged cells. Various molecules take part in apoptosis, main participants are Bcl-2 protein family and caspases. The latter one are responsible for apoptosis execution, while Bcl-2 protein family regulates apoptotic pathway. Failure of this regulation may cause several pathologies, including development of neoplastic tissue. Human endometrium is a speci c tissue, in which apoptosis is present in cycling pattern. Present study shows expression level of Bcl-2, Bax, Bad, Bid, pro-caspase-3, caspase-3 and PARP in normal, atrophic, hyperplastic and cancerous (Grade I and II) human endometrium. Bad and Bid proteins can be possible breakpoints in neoplastic transfer due to opposit expression in cancerous and hyperplastic endometrium.