Antigenic and enzymatic properties of sand fly saliva

Abstract

i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...

i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...