Antigenic and enzymatic properties of sand fly saliva
Antigenic and enzymatic properties of sand fly saliva
dissertation thesis (DEFENDED)
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http://hdl.handle.net/20.500.11956/18282Identifiers
Study Information System: 112579
Collections
- Kvalifikační práce [19113]
Author
Advisor
Consultant
Kopecký, Jan
Referee
Salát, Jiří
Bilej, Martin
Faculty / Institute
Faculty of Science
Discipline
-
Department
Department of Parasitology
Date of defense
16. 9. 2008
Publisher
Univerzita Karlova, Přírodovědecká fakultaLanguage
English
Grade
Pass
i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...
i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...