The Biochemistry of Zine and Iron Proteomic Studies

Abstract

This thesis, The Biochemistry of Zinc and Iron - Proteomic Studies, consists of five sections. We were studying the biochemistry of two different metal elements - iron and zinc, therefore the thesis has two main aims (iron and zinc related) and each of the experimental sections are divided into two logical subsections A and B. The first section - Introduction includes a general introduction reviewing the current knowledge about the biochemistry of iron and zinc and it also specifies the objectives of this study. The first part of the Introduction is a review of iron biochemistry. It has following seven chapters: General properties of iron; Iron absorption, storage and toxicity in human; Iron transport and homeostasis; Iron regulatory proteins - IRP/IRE system; Mitochondrial iron metabolism; Liver and iron and Iron disorders. In the second part of the Introduction, the biochemistry of zinc is reviewed in these eight chapters: Zinc - general properties; Zinc transporters; Zinc finger proteins; The role of zinc fingers in leukemogenesis; Functions of zinc in cellular signalling; Regulatory functions of zinc in cell proliferation; Role for Zinc in cell differentiation and apoptosis; Zinc - remarks and perspectives. The third part of the Introduction introduces the specific objectives of this thesis. It gives a...