dc.contributor.advisor | Mokrý, Jaroslav | |
dc.creator | Pisal, Rishikaysh | |
dc.date.accessioned | 2018-07-09T10:04:59Z | |
dc.date.available | 2018-07-09T10:04:59Z | |
dc.date.issued | 2018 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11956/99416 | |
dc.description.abstract | Cellular reprogramming as a tool for harvesting patient specific stem cells In the year 2006, Dr. Yamanaka surprised the entire field of medicine, by reporting a technique of inducing pluripotency in somatic cells. In his article, he had displayed that fibroblasts could be reprogrammed to pluripotent stem cell state, by ectopic expression of four transcription factors namely OCT4, SOX2, c-MYC and KLF4. His discovery made a paradigm shift in the field of reprogramming because previous methods of reprogramming were dependent on use of human oocytes and this raised ethical concerns. Moreover, his technique of cellular reprograming broadened the spectrum of application of somatic cells in regenerative medicine. Objectives of my research were focused on; development of an optimised protocol for detection of mycoplasma that commonly infects animal tissue culture; detailed characterization of reprogrammed clones; targeted differentiation of iPSC towards myogenic lineage, and construction of an expression vector, optimised for miRNA expression. For detecting mycoplasma infection, we adapted the protocol of Uphoff et al. (2002). By skipping the DNA extraction step (reported in the original protocol) and instead directly using cell culture supernatant and a robust polymerase enzyme for performing PCR, we... | en_US |
dc.language | English | cs_CZ |
dc.language.iso | en_US | |
dc.publisher | Univerzita Karlova, Lékařská fakulta v Hradci Králové | cs_CZ |
dc.title | Cellular Reprogramming as a Tool for Harvesting Patient-specific Stem Cells | en_US |
dc.type | dizertační práce | cs_CZ |
dcterms.created | 2018 | |
dcterms.dateAccepted | 2018-06-18 | |
dc.description.department | Department of Histology and Embryology | en_US |
dc.description.department | Ústav histologie a embryologie | cs_CZ |
dc.description.faculty | Faculty of Medicine in Hradec Králové | en_US |
dc.description.faculty | Lékařská fakulta v Hradci Králové | cs_CZ |
dc.identifier.repId | 133024 | |
dc.title.translated | Buněčné reprogramování jako nástroj pro získání kmenových buněk od pacienta | cs_CZ |
dc.contributor.referee | Hampl, Aleš | |
dc.contributor.referee | Jendelová, Pavla | |
thesis.degree.name | Ph.D. | |
thesis.degree.level | doktorské | cs_CZ |
thesis.degree.discipline | - | en_US |
thesis.degree.discipline | - | cs_CZ |
thesis.degree.program | Anatomie, histologie a embryologie | cs_CZ |
thesis.degree.program | Anatomy, Histology and Embryology | en_US |
uk.thesis.type | dizertační práce | cs_CZ |
uk.taxonomy.organization-cs | Lékařská fakulta v Hradci Králové::Ústav histologie a embryologie | cs_CZ |
uk.taxonomy.organization-en | Faculty of Medicine in Hradec Králové::Department of Histology and Embryology | en_US |
uk.faculty-name.cs | Lékařská fakulta v Hradci Králové | cs_CZ |
uk.faculty-name.en | Faculty of Medicine in Hradec Králové | en_US |
uk.faculty-abbr.cs | LFHK | cs_CZ |
uk.degree-discipline.cs | - | cs_CZ |
uk.degree-discipline.en | - | en_US |
uk.degree-program.cs | Anatomie, histologie a embryologie | cs_CZ |
uk.degree-program.en | Anatomy, Histology and Embryology | en_US |
thesis.grade.cs | Prospěl | cs_CZ |
thesis.grade.en | Pass | en_US |
uk.abstract.en | Cellular reprogramming as a tool for harvesting patient specific stem cells In the year 2006, Dr. Yamanaka surprised the entire field of medicine, by reporting a technique of inducing pluripotency in somatic cells. In his article, he had displayed that fibroblasts could be reprogrammed to pluripotent stem cell state, by ectopic expression of four transcription factors namely OCT4, SOX2, c-MYC and KLF4. His discovery made a paradigm shift in the field of reprogramming because previous methods of reprogramming were dependent on use of human oocytes and this raised ethical concerns. Moreover, his technique of cellular reprograming broadened the spectrum of application of somatic cells in regenerative medicine. Objectives of my research were focused on; development of an optimised protocol for detection of mycoplasma that commonly infects animal tissue culture; detailed characterization of reprogrammed clones; targeted differentiation of iPSC towards myogenic lineage, and construction of an expression vector, optimised for miRNA expression. For detecting mycoplasma infection, we adapted the protocol of Uphoff et al. (2002). By skipping the DNA extraction step (reported in the original protocol) and instead directly using cell culture supernatant and a robust polymerase enzyme for performing PCR, we... | en_US |
uk.file-availability | V | |
uk.publication.place | Hradec Králové | cs_CZ |
uk.grantor | Univerzita Karlova, Lékařská fakulta v Hradci Králové, Ústav histologie a embryologie | cs_CZ |