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Buněčné reprogramování jako nástroj pro získání kmenových buněk od pacienta
dc.contributor.advisorMokrý, Jaroslav
dc.creatorPisal, Rishikaysh
dc.date.accessioned2018-07-09T10:04:59Z
dc.date.available2018-07-09T10:04:59Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/20.500.11956/99416
dc.description.abstractCellular reprogramming as a tool for harvesting patient specific stem cells In the year 2006, Dr. Yamanaka surprised the entire field of medicine, by reporting a technique of inducing pluripotency in somatic cells. In his article, he had displayed that fibroblasts could be reprogrammed to pluripotent stem cell state, by ectopic expression of four transcription factors namely OCT4, SOX2, c-MYC and KLF4. His discovery made a paradigm shift in the field of reprogramming because previous methods of reprogramming were dependent on use of human oocytes and this raised ethical concerns. Moreover, his technique of cellular reprograming broadened the spectrum of application of somatic cells in regenerative medicine. Objectives of my research were focused on; development of an optimised protocol for detection of mycoplasma that commonly infects animal tissue culture; detailed characterization of reprogrammed clones; targeted differentiation of iPSC towards myogenic lineage, and construction of an expression vector, optimised for miRNA expression. For detecting mycoplasma infection, we adapted the protocol of Uphoff et al. (2002). By skipping the DNA extraction step (reported in the original protocol) and instead directly using cell culture supernatant and a robust polymerase enzyme for performing PCR, we...en_US
dc.languageEnglishcs_CZ
dc.language.isoen_US
dc.publisherUniverzita Karlova, Lékařská fakulta v Hradci Královécs_CZ
dc.titleCellular Reprogramming as a Tool for Harvesting Patient-specific Stem Cellsen_US
dc.typedizertační prácecs_CZ
dcterms.created2018
dcterms.dateAccepted2018-06-18
dc.description.departmentDepartment of Histology and Embryologyen_US
dc.description.departmentÚstav histologie a embryologiecs_CZ
dc.description.facultyFaculty of Medicine in Hradec Královéen_US
dc.description.facultyLékařská fakulta v Hradci Královécs_CZ
dc.identifier.repId133024
dc.title.translatedBuněčné reprogramování jako nástroj pro získání kmenových buněk od pacientacs_CZ
dc.contributor.refereeHampl, Aleš
dc.contributor.refereeJendelová, Pavla
thesis.degree.namePh.D.
thesis.degree.leveldoktorskécs_CZ
thesis.degree.discipline-en_US
thesis.degree.discipline-cs_CZ
thesis.degree.programAnatomie, histologie a embryologiecs_CZ
thesis.degree.programAnatomy, Histology and Embryologyen_US
uk.thesis.typedizertační prácecs_CZ
uk.taxonomy.organization-csLékařská fakulta v Hradci Králové::Ústav histologie a embryologiecs_CZ
uk.taxonomy.organization-enFaculty of Medicine in Hradec Králové::Department of Histology and Embryologyen_US
uk.faculty-name.csLékařská fakulta v Hradci Královécs_CZ
uk.faculty-name.enFaculty of Medicine in Hradec Královéen_US
uk.faculty-abbr.csLFHKcs_CZ
uk.degree-discipline.cs-cs_CZ
uk.degree-discipline.en-en_US
uk.degree-program.csAnatomie, histologie a embryologiecs_CZ
uk.degree-program.enAnatomy, Histology and Embryologyen_US
thesis.grade.csProspělcs_CZ
thesis.grade.enPassen_US
uk.abstract.enCellular reprogramming as a tool for harvesting patient specific stem cells In the year 2006, Dr. Yamanaka surprised the entire field of medicine, by reporting a technique of inducing pluripotency in somatic cells. In his article, he had displayed that fibroblasts could be reprogrammed to pluripotent stem cell state, by ectopic expression of four transcription factors namely OCT4, SOX2, c-MYC and KLF4. His discovery made a paradigm shift in the field of reprogramming because previous methods of reprogramming were dependent on use of human oocytes and this raised ethical concerns. Moreover, his technique of cellular reprograming broadened the spectrum of application of somatic cells in regenerative medicine. Objectives of my research were focused on; development of an optimised protocol for detection of mycoplasma that commonly infects animal tissue culture; detailed characterization of reprogrammed clones; targeted differentiation of iPSC towards myogenic lineage, and construction of an expression vector, optimised for miRNA expression. For detecting mycoplasma infection, we adapted the protocol of Uphoff et al. (2002). By skipping the DNA extraction step (reported in the original protocol) and instead directly using cell culture supernatant and a robust polymerase enzyme for performing PCR, we...en_US
uk.file-availabilityV
uk.publication.placeHradec Královécs_CZ
uk.grantorUniverzita Karlova, Lékařská fakulta v Hradci Králové, Ústav histologie a embryologiecs_CZ


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