Molecular dynamics of proteins interacting with substrate or ligand: mitochondrial processing peptidase and FixL oxygen sensor
Molekulární dynamika proteinů integrujících s ligandem nebo substrátem: mitochondriální procesující peptidáza a FixL kyslíkový senzor
dizertační práce (OBHÁJENO)
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Trvalý odkaz
http://hdl.handle.net/20.500.11956/95146Identifikátory
SIS: 112355
Kolekce
- Kvalifikační práce [19966]
Autor
Vedoucí práce
Oponent práce
Váchová, Libuše
Branny, Pavel
Fakulta / součást
Přírodovědecká fakulta
Obor
Molekulární a buněčná biologie
Katedra / ústav / klinika
Katedra genetiky a mikrobiologie
Datum obhajoby
27. 9. 2007
Nakladatel
Univerzita Karlova, Přírodovědecká fakultaJazyk
Angličtina
Známka
Prospěl
I I Ovenvrew I I I II I I I I I I I I I The presenteddoctoralthesis includesfive publishedscientificarticlesand one manuscriptpreparedfor submission.All describestudieson threeproteinmodels.The four papersnumbered(1)- (4)inthelistofpublications,sharea commonobjectivee.i.observingand describingfunctionalproteindynamicsandconformationchangeinducedby ligandor substrate binding,andrepresentthemainresultofmyPhDwork. Thepapers(1)and(4)offerresultsof a projectfrommyhomelaboratoryattheInstitute ofMicrobiologyAS CR, LaboratoryforBiologyofSecondaryMetabolism,underthesupervisionof JiřÍ Janata, PhD. The projectis focusedon the protein-proteindynamicsinteractionof mitochondrialprocessingpeptidase(MPP) fromSaccharomycescerevisiaewithits preproteins substrates. The papers(2)and(3)describeresultsof a project,inwhichI haveparticipatedduring myMarieCuriefellowshipattheEcolePolytechnique(PalaiseauCedex,France),Laboratoryfor OpticsandBiosciences,in2004.Theprojectconcernsresearchon proteinstructuraldynamicsof theheme-basedoxygensensorFixLfromBradyrhizobiumjaponicum,inwhichoxygenbindingto the heme sensor domain inducesconformationchange,which regulatesthe activityof neighboringkinasedomain. ln bothprojects,analogyin methodicalapproach,i.e.seriesof molecularbiologyand...
I I Ovenvrew I I I II I I I I I I I I I The presenteddoctoralthesis includesfive publishedscientificarticlesand one manuscriptpreparedfor submission.All describestudieson threeproteinmodels.The four papersnumbered(1)- (4)inthelistofpublications,sharea commonobjectivee.i.observingand describingfunctionalproteindynamicsandconformationchangeinducedby ligandor substrate binding,andrepresentthemainresultofmyPhDwork. Thepapers(1)and(4)offerresultsof a projectfrommyhomelaboratoryattheInstitute ofMicrobiologyAS CR, LaboratoryforBiologyofSecondaryMetabolism,underthesupervisionof JiřÍ Janata, PhD. The projectis focusedon the protein-proteindynamicsinteractionof mitochondrialprocessingpeptidase(MPP) fromSaccharomycescerevisiaewithits preproteins substrates. The papers(2)and(3)describeresultsof a project,inwhichI haveparticipatedduring myMarieCuriefellowshipattheEcolePolytechnique(PalaiseauCedex,France),Laboratoryfor OpticsandBiosciences,in2004.Theprojectconcernsresearchon proteinstructuraldynamicsof theheme-basedoxygensensorFixLfromBradyrhizobiumjaponicum,inwhichoxygenbindingto the heme sensor domain inducesconformationchange,which regulatesthe activityof neighboringkinasedomain. ln bothprojects,analogyin methodicalapproach,i.e.seriesof molecularbiologyand...