Th1/Th2 genový polymorfismus cytokinů u pacientů s idiopatickou plicní fibrózou
Th1/Th2 cytokine gene polymorphisms in patients with idiopathic pulmonary fibrosis
dizertační práce (OBHÁJENO)
Zobrazit/ otevřít
Trvalý odkaz
http://hdl.handle.net/20.500.11956/9362Identifikátory
SIS: 123129
Kolekce
- Kvalifikační práce [4306]
Autor
Vedoucí práce
Oponent práce
Posová, Helena
Šedivá, Anna
Fakulta / součást
1. lékařská fakulta
Obor
-
Katedra / ústav / klinika
Institut klinické a experimentální medicíny
Datum obhajoby
21. 5. 2007
Nakladatel
Univerzita Karlova, 1. lékařská fakultaJazyk
Čeština
Známka
Prospěl/a
Objectives: Idiopathic pulmonary fibrosis (IPF) is a serious disease characterized with progressive scarring of the lungs in which the genetic background is supposed. The aim of our study was to investigate Th1/Th2 cytokine gene polymorphisms to evaluate their possible influence on IPF development. Then we have correlated selected polymorphisms of IL-1, IL-4 and IL-12 groups ( the selection was based on our previous results) with clinical parameters and high resolution computed tomography (HRCT) as a markers of disease stage and progression. Methods: We investigated 30 patients with IPF and 103 healthy volunteers for the cytokines polymorphisms of the IL-1 alpha, IL-1 beta, IL-1R, IL-1RA, IL-2, IL-4, IL-6, IL-10, IL-12, TNF alpha, IFN gamma, TGF beta, IL-1 beta, IL-2, IL-4 and IL-4RA genes. The PCR-SSP method was used for measurement. Then the correlations of vital capacity(VC) and diffusing capacity for carbon monoxide(DLCO), bronchoalveolar lavage (BAL) fluid cell counts and high resolution computed tomography (HRCT) alveolar and interstitial scores with different genotypes of groups of IL-1, IL-4, and IL-12 cytokines and their receptor antagonists. The HRCT results were evaluated by an experienced viewer using the interstitial and alveolar score scales, which were based on the IPF HRCT description system...