Mikroanatomické a mikrobiologické aspekty konzumace mikromycet druhem Tyrophagus putrescentiae (Acari: Acaridida)
Consumption of some micromycetes by Tyrophagus putrescentiae (Acari: Acaridida) and its microanatomical and microbiological characteristics
diploma thesis (DEFENDED)
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http://hdl.handle.net/20.500.11956/93447Identifiers
Study Information System: 61911
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- Kvalifikační práce [20083]
Author
Advisor
Referee
Hubert, Jan
Faculty / Institute
Faculty of Science
Discipline
Biology
Department
Department of Zoology
Date of defense
22. 9. 2008
Publisher
Univerzita Karlova, Přírodovědecká fakultaLanguage
Czech
Grade
Excellent
Mycophagy should not be considered as a single and homogeneous category of nutritional biology due to the specific symbiotic chitinolytic bacteria associated with mites and fungi. To test interaction amongmites, fungi, and chitinolytic bacteria, experiments were conducted on the model species Tyrophagus putrescentiae (Schrank). Mucor sp, Alternaria alternata, Penicillium claviforme, P. griseofulvum, and Verticillium sp. were plated onto malt agar and offeredto T. putrescentiae in the laboratory. Mites were evaluated utilizing microanatomical examination based on histology, excrement analysis using fluorescence microscopy, bacterial plating, impact of mite homogenate on fungi in Petri dishes, reproduction of mites feeding upon each fungus, and isolation of associated bacteria inside mites. There were clear differences regarding the digested spores of different fungi passing through the gut and subsequently in the feces. Abundances of bacterial cells in excrement also corresponded to the fungi offered. The extracts from mites had chitinolytic activity, and the plated bacteria are known to produce exochitinases. The various feeding patterns observed were caused by differences in the cell wall structures of the tested fungi. The study illustrates that mycophagy in saprophagous mites does not consist of...