Phylogenetic relationships within the Gobius-lineage (Gobiidae)

Abstract

1 1 Supplement The content of the supplement: p. 1: Table 15: A list of species represented in this work and their description p. 4: Table 16: Mean genetic distances (p-distance) on cyt b within the Gobius-lineage (%) p. 5: Table 17: Mean genetic distances (p-distance) on COI within the Gobius-lineage (%) p. 6: Table 18: Mean genetic distances (p-distance) on Rh within the Gobius-lineage (%) p. 7: Table 19: Mean genetic distances (p-distance) on RAG within the Gobius-lineage (%) Species Description Aphia minuta (Risso, 1810) Apogon imberbis (Linnaeus, 1758) Apogon maculatus (Poey, 1860) Babka gymnotrachelus (Kessler, 1857) Bathygobius soporator (Valenciennes, 1837) Benthophilus abdurahmanovi Ragimov, 1978 Benthophilus granulosus Kessler, 1877 Benthophilus mahmudbejovi Ragimov, 1976 Benthophilus stellatus (Sauvage, 1874) Bostrychus sinensis Lacepéde, 1871 Buenia affinis Iljin, 1930 Caspiosoma caspium (Kessler, 1877) cf Gnatholepis thompsoni Jordan, 1904 Corcyrogobius liechtensteini (Kolombatovic, 1891) Corcyrogobius lubbocki Miller, 1988 Coryogalops anomolus Smith, 1958 Crystallogobius linearis (Düben, 1854) Deltentosteus collonianus (Risso, 1820) Didogobius splechtnai Ahnelt&Patzner, 1995 Didogobius wirtzi Schlieven&Kovačić, 2008 Eleotris fusca (Forster, 1801) Gammogobius steinitzi Bath, 1971 Gnatholepis...

Gobiidae is one of the largest families of teleost fishes with nearly 2000 species currently recognized. They have a worldwide distribution with exception of Arctic and Antarctic areas, inhabiting marine, brackish and freshwaters. Mostly, they are small-sized and live inconspicuously on the bottom. Their phylogeny has been studied only partially. In Europe, three independent lineages of gobies exist (Gobius-, Aphia- and Pomatoschistus-lineage), most of the species of these lineages are marine. In this work, I performed a multilocus study of the Gobius-lineage encompassing the majority of the species. Mitochondrial (cytochrome b and cytochrome c oxidase I) as well as nuclear (rhodopsin and recombination activating gene) markers were used. 480 individuals of 30 species were analyzed in the laboratory and sequences of further 25 - 32 species were downloaded from the Genbank and added to a dataset of each marker according to availability. Mitochondrial markers were more informative than the nuclear ones. The usefulness of cytochrome c oxidase I for studying phylogenetic relationships of gobies was compared with cytochrome b. Cytochrome c oxidase I showed to be useful for identification of the species, but has some limitations in resolving deeper phylogenetic relationships in gobies. Cytochrome b showed...