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The study of the biosynthetic pathway of the antibiotic lincomycin
dc.contributor.advisorSpížek, Jaroslav
dc.creatorNovotná, Jitka
dc.date.accessioned2019-05-03T13:37:48Z
dc.date.available2019-05-03T13:37:48Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/20.500.11956/6691
dc.description.abstract1. L.3'4-Dihydroxyphenyl a|anine.extraďo|cleavage cyclizationin lincomycin biosynthesis. is followed by intra-molecular The aim of the work gene,characterizeit better DOPA aromaticring is the lincomycinsynthesis. was to assignfunctionto the proteincodedfor by an lmbBl and confirm the assumptionthat2,3-extradiolfission of the actualreactioninvolvedin the metabolicpathwayleadingto fooH HrN-) aOH tyÍo8|n cooH cooH ,,"1 ,,"4 .Ť:+*.i'} 2'3.6ÓcoDoPA /t{3€Íboxy+ox}prcponý}2'34|hyÚ} 1/í pyÍrolc2€íboxy|lo rc|d Fig' 1 |nitia|steps of the amino acid subpathway oÍthe |incomycin biosynthesis The resultsof the feedingexperimentswith labeledintermediatesand subsequent NMR analysis(BRAHME etal., 1984),bearedwitnessof thefactthattheaminoacid sub- pathwayof thelíncomycinbiosynthesisincludes2,3-extradiolcleavageof DoPA (Fig. 1). Neusser and coworkers (NEUSSER et al., 1998) showed that LmbBl catalyzes conversionof DOPA to anunspecifiedyellowcompound. It appearedinapplicableto isolatethe LmbBl reactionproductdirectlyfrom in y,itroreacÍioncatalyzedby thepurifiedLmbBl partlyas LmbBl lost mostof its activity duringdialysis.mostprobablydue to oxidationof theferrousion proposedas a cofactor, andpartlydue to thefact thatmanydiÍ.ferentDoPA oxidationproductswereproducedin the system.Instead,a system simrlar to that applied Íbr...en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleStudium biosyntetické dráhy antibiotika linkomycinucs_CZ
dc.typedizertační prácecs_CZ
dcterms.created2008
dcterms.dateAccepted2008-09-26
dc.description.departmentDepartment of Genetics and Microbiologyen_US
dc.description.departmentKatedra genetiky a mikrobiologiecs_CZ
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId112684
dc.title.translatedThe study of the biosynthetic pathway of the antibiotic lincomycinen_US
dc.contributor.refereeWeiser, Jaroslav
dc.contributor.refereeGašparík, Juraj
dc.identifier.aleph001003237
thesis.degree.namePh.D.
thesis.degree.leveldoktorskécs_CZ
thesis.degree.discipline-cs_CZ
thesis.degree.discipline-en_US
thesis.degree.programMikrobiologiecs_CZ
thesis.degree.programMicrobiologyen_US
uk.thesis.typedizertační prácecs_CZ
uk.taxonomy.organization-csPřírodovědecká fakulta::Katedra genetiky a mikrobiologiecs_CZ
uk.taxonomy.organization-enFaculty of Science::Department of Genetics and Microbiologyen_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.cs-cs_CZ
uk.degree-discipline.en-en_US
uk.degree-program.csMikrobiologiecs_CZ
uk.degree-program.enMicrobiologyen_US
thesis.grade.csProspěl/acs_CZ
thesis.grade.enPassen_US
uk.abstract.en1. L.3'4-Dihydroxyphenyl a|anine.extraďo|cleavage cyclizationin lincomycin biosynthesis. is followed by intra-molecular The aim of the work gene,characterizeit better DOPA aromaticring is the lincomycinsynthesis. was to assignfunctionto the proteincodedfor by an lmbBl and confirm the assumptionthat2,3-extradiolfission of the actualreactioninvolvedin the metabolicpathwayleadingto fooH HrN-) aOH tyÍo8|n cooH cooH ,,"1 ,,"4 .Ť:+*.i'} 2'3.6ÓcoDoPA /t{3€Íboxy+ox}prcponý}2'34|hyÚ} 1/í pyÍrolc2€íboxy|lo rc|d Fig' 1 |nitia|steps of the amino acid subpathway oÍthe |incomycin biosynthesis The resultsof the feedingexperimentswith labeledintermediatesand subsequent NMR analysis(BRAHME etal., 1984),bearedwitnessof thefactthattheaminoacid sub- pathwayof thelíncomycinbiosynthesisincludes2,3-extradiolcleavageof DoPA (Fig. 1). Neusser and coworkers (NEUSSER et al., 1998) showed that LmbBl catalyzes conversionof DOPA to anunspecifiedyellowcompound. It appearedinapplicableto isolatethe LmbBl reactionproductdirectlyfrom in y,itroreacÍioncatalyzedby thepurifiedLmbBl partlyas LmbBl lost mostof its activity duringdialysis.mostprobablydue to oxidationof theferrousion proposedas a cofactor, andpartlydue to thefact thatmanydiÍ.ferentDoPA oxidationproductswereproducedin the system.Instead,a system simrlar to that applied Íbr...en_US
uk.file-availabilityP
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra genetiky a mikrobiologiecs_CZ
thesis.grade.codeP
dc.identifier.lisID990010032370106986


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