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The Role of WT1 gene in normal hematopoiesis and leukemogenesis
dc.contributor.advisorTrka, Jan
dc.creatorKramarzová, Karolina
dc.date.accessioned2017-04-12T14:59:38Z
dc.date.available2017-04-12T14:59:38Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/20.500.11956/5514
dc.description.abstract6 Abstract The Wilms' tumor gene is highly expressed in a large proportion of human acute leukemias and other hematological malignancies. It is thought to play an important role in human hematpoiesis and leukemogenesis. Despite the large number of studies, WT1 expression patterns and its clinical significance in acute myeloid leukemia remain still controversial. To investigate the prognostic relevance of initial WT1 expression and its usefulness as a marker for minimal residual disease, we have analysed 66 bone marrow samples from newly diagnosed AML patients. RQ-RT-PCR for absolute quantification of total WT1 was designed according to Europe Against Cancer Program. In 82 % of samples we detected a higher expression of WT1 compared to normal healthy donors or patients with acute lymphoblastic leukemia (p<0.0001). We did not find any correlation between initial WT1 expression level and age or sex. Patients with FAB M3 subtype showed significantly higher WT1 levels than other subtypes, especially M5 (p<0.001). We found a strikingly high WT1 expression in standard risk group patients compared to high risk group (p<0.0007). Children with FAB/cytogenetical favorable prognostic factors have high WT1 expression, while there was no relation between WT1 levels at diagnosis and day 15 BM response. We did not find any...en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleRole genu WT1 v normální hematopoese a leukemogenesecs_CZ
dc.typediplomová prácecs_CZ
dcterms.created2008
dcterms.dateAccepted2008-09-22
dc.description.departmentKatedra genetiky a mikrobiologiecs_CZ
dc.description.departmentDepartment of Genetics and Microbiologyen_US
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId52763
dc.title.translatedThe Role of WT1 gene in normal hematopoiesis and leukemogenesisen_US
dc.contributor.refereeZadražil, Stanislav
dc.identifier.aleph000999430
thesis.degree.nameMgr.
thesis.degree.levelnavazující magisterskécs_CZ
thesis.degree.disciplineGenetika, molekulární biologie a virologiecs_CZ
thesis.degree.disciplineGenetics, Molecular Biology and Virologyen_US
thesis.degree.programBiologiecs_CZ
thesis.degree.programBiologyen_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.csGenetika, molekulární biologie a virologiecs_CZ
uk.degree-discipline.enGenetics, Molecular Biology and Virologyen_US
uk.degree-program.csBiologiecs_CZ
uk.degree-program.enBiologyen_US
thesis.grade.csVýborněcs_CZ
thesis.grade.enExcellenten_US
uk.abstract.en6 Abstract The Wilms' tumor gene is highly expressed in a large proportion of human acute leukemias and other hematological malignancies. It is thought to play an important role in human hematpoiesis and leukemogenesis. Despite the large number of studies, WT1 expression patterns and its clinical significance in acute myeloid leukemia remain still controversial. To investigate the prognostic relevance of initial WT1 expression and its usefulness as a marker for minimal residual disease, we have analysed 66 bone marrow samples from newly diagnosed AML patients. RQ-RT-PCR for absolute quantification of total WT1 was designed according to Europe Against Cancer Program. In 82 % of samples we detected a higher expression of WT1 compared to normal healthy donors or patients with acute lymphoblastic leukemia (p<0.0001). We did not find any correlation between initial WT1 expression level and age or sex. Patients with FAB M3 subtype showed significantly higher WT1 levels than other subtypes, especially M5 (p<0.001). We found a strikingly high WT1 expression in standard risk group patients compared to high risk group (p<0.0007). Children with FAB/cytogenetical favorable prognostic factors have high WT1 expression, while there was no relation between WT1 levels at diagnosis and day 15 BM response. We did not find any...en_US
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra genetiky a mikrobiologiecs_CZ


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