Voltametrické stanovení genotoxického 6-nitrochrysenu na rtuťovým meniskem modifikované stříbrné pevné amalgámové elektrodě
Voltammetric Determination of Genotoxic 6-Nitrochrysene at a Mercury Meniscus Modified Silver Solid Amalgam Electrode
bachelor thesis (DEFENDED)

View/ Open
Permanent link
http://hdl.handle.net/20.500.11956/38686Identifiers
Study Information System: 89345
CU Caralogue: 990013707410106986
Collections
- Kvalifikační práce [20864]
Author
Advisor
Referee
Fischer, Jan
Faculty / Institute
Faculty of Science
Discipline
Chemistry in Natural Sciences
Department
Department of Analytical Chemistry
Date of defense
21. 6. 2011
Publisher
Univerzita Karlova, Přírodovědecká fakultaLanguage
Czech
Grade
Excellent
Keywords (Czech)
6-nitrochrysen, DC voltametrie, diferenční pulzní voltametrie, stříbrná pevná amalgámová elektroda, UV-VIS absorpční spektrofotometrieKeywords (English)
6-Nitrochrysene, DC Voltammetry, Differential Pulse Voltammetry, Silver Solid Amalgam Electrode, UV-VIS absorption SpectrophotometryThis Bachelor Thesis is devoted to the study of electrochemical behavior of 6-nitrochrysene (6-NCH) and to the finding of the optimum conditions for its determination using direct current voltammetry (DCV) and differential pulse voltammetry (DPV) at a mercury meniscus modified silver solid amalgam electrode (m-AgSAE). For measuring the concentration dependences of 6-NCH, the optimum medium methanol - BR buffer pH 9.0 (1:1) and the proper regeneration potentials Ereg,1 = 0 mV, Ereg,2 = -600 mV have been chosen for DCV at m-AgSAE and the medium methanol - BR buffer pH 10.0 (1:1) and the proper regeneration potentials Ereg,1 = 0 mV, Ereg,2 = -750 mV have been chosen for DPV at m-AgSAE. The concentration dependence was found to be linear only for the lowest concentration range of 10-7 mol·l-1 . In the concentration range of 10-6 mol·l-1 , the concentration dependences observed were polynomial. This result indicates a possible adsorption of 6-NCH on the surface of working electrode. Reached limits of quantification (LQ) were 1·10-7 mol·l-1 for DCV at m-AgSAE and 5·10-8 mol·l-1 for DPV at m-AgSAE. For comparison, the concentration dependence of 6-NCH was measured in methanol using UV-VIS spectrophotometry. Reached LQ ~ 1·10-7 mol·l-1 is comparable with LQs reached using DCV and DPV techniques.