Voltametrické stanovení genotoxického 6-nitrochrysenu na rtuťovým meniskem modifikované stříbrné pevné amalgámové elektrodě

Abstract

This Bachelor Thesis is devoted to the study of electrochemical behavior of 6-nitrochrysene (6-NCH) and to the finding of the optimum conditions for its determination using direct current voltammetry (DCV) and differential pulse voltammetry (DPV) at a mercury meniscus modified silver solid amalgam electrode (m-AgSAE). For measuring the concentration dependences of 6-NCH, the optimum medium methanol - BR buffer pH 9.0 (1:1) and the proper regeneration potentials Ereg,1 = 0 mV, Ereg,2 = -600 mV have been chosen for DCV at m-AgSAE and the medium methanol - BR buffer pH 10.0 (1:1) and the proper regeneration potentials Ereg,1 = 0 mV, Ereg,2 = -750 mV have been chosen for DPV at m-AgSAE. The concentration dependence was found to be linear only for the lowest concentration range of 10-7 mol·l-1 . In the concentration range of 10-6 mol·l-1 , the concentration dependences observed were polynomial. This result indicates a possible adsorption of 6-NCH on the surface of working electrode. Reached limits of quantification (LQ) were 1·10-7 mol·l-1 for DCV at m-AgSAE and 5·10-8 mol·l-1 for DPV at m-AgSAE. For comparison, the concentration dependence of 6-NCH was measured in methanol using UV-VIS spectrophotometry. Reached LQ ~ 1·10-7 mol·l-1 is comparable with LQs reached using DCV and DPV techniques.