Oxidační stres navozený železem a jeho ovlivnění flavonoidy a bisfosfonáty

Abstract

Iron is an essential element for living organisms. However, as it is a transition metal, it can participate in Fenton reaction resulting in generation of free radicals and oxidative damage to tissues. Antioxidants may prevent possible iron toxicity by chelating free iron or scavenging free radicals. Falvonoids are naturally occurring substances that are capable of formation of complexes with metals, including iron. T h e y have been show to possess antioxidant activity, which depends on molecular complexity of numerous types of flavonoids, e.g. quercetin and silibinin. Bisphosphonates are synthetic drugs used to treat various metabolic diseases of bones. Their principál effect is an inhibition of osteclast activity leading to a decreased bone resorption. Bisphosphonates have been however shown to exert some antioxidant activity in in vitro experiments, too. The aim of this PhD thesis was to investigate the role of iron in toxicity of other metals (cadmium) and the effect of flavonoids (quercetin and silibinin) and bisphosphonates (clodronate, etidronate and risedronate) on iron-induced oxidative damage in vivo. Experiments were performed in male mice (CD-1, Charles River, 25-35 body weight). Iron was administered intraperitoneally or in the diet. Cadmium was administered subcutaneously. Flavonoids and...

Autor ve své disertační práci zkoumal úlohu železa v toxicitě jiných kovů (kadmium ) a vliv flavonoidů a bisfosfonátů na železo indukované oxidačním poškozením in vivo . Experimenty byly provedeny u myších samců ( CD - 1 , Charles River , 25-35 tělesné hmotnosti ) . Dávka byla prokázána . Vysoké dávky vedly k oxidačnímu poškození ( lipoperoxidace indukční a snížení hladiny glutathionu ) , zatímco nižší dávka podávána po dlouhou dobu ( 7 týdnů) měla antioxidační účinek ( nižší lipoperoxidace a zvýšená aktivita glutathion peroxidáza ) .