Prospektivní studie role oxidačního stresu u akutních intoxikací metanolem.

Abstract

4 SOUHRN PR CE V E TIN Kontext: Ak n in ikace me an lem h je l ka na i . Me an l e organismu me ab li je na f maldeh d a k elin m a en , k e inhib je c ch m c id mi ch nd i ch a m e d l na ji ida n h e . Cíl: Zjistit, jak je le ida n h e a gene i ak n h ne n ln h k en cen ln h ne h m (CNS) ji dl h d b ch n ledk a me an lem a ch nick ch ne degene a i n ch ce le ech n led j c ch ak n e ici me an l . Materi l a metodika: Intoxikace metanolem byla potvrzena analyticky u 55 pacient a a en ch d b die, jejich k d b a b l 46,7 3,6 le (9 en, 46 m ). V ichni acien i b li le n 41 k n lami et eni v r mci prospektivn longitudin ln kohortov die. P i jm d nem cnice, b hem h i ali ace a a ideln ch in e alech n b l acien m deb n k k e n h a, e k e ch b l an n k ncen ace ma ke ida i n h k en li id 4-hydroxy-trans-2-hexenal (HHE), 4-hydroxynonenal (HNE), malondialdehyd (MDA), 8-izoprostan, n kle ch k elin 8-hydroxy-2 -deoxyguanosin (8-OHdG), 8-hydroxyguanosin (8-OHG), 5- (hydroxymethyl)uracil (5-OHMU), ein h -tyrosin (o-Tyr), nitrotyrosin (NOTyr), chlorotyrosin (Cl-T ) a le k ien LTB4, LTC4, LTD4, LTE4. Pacien i b li d le e ni 4,9 0,6, 25,0 0,6 a 49,9 0,5 m s c po dimisi z nemocnice. Protokol klinick ho vy et en ah n al bi chemick lab a n e en , e en n a ne l gick , m en zrakov ch evokovan ch potenci l (VEP), ick k he en n m...

5 SUMMARY Context: Acute methanol poisoning is a life-threatening condition. Methanol is metabolized in the organism to formaldehyde and than to formic acid, which inhibits cytochrome c oxidase in mitochondria and thus contributes to the development of oxidative stress. Aim: To study the role of oxidative stress in the pathogenesis of acute neuronal damage to the central nervous system (CNS), in the development of long-term sequelae of methanol poisoning and chronic neurodegenerative processes in the years following acute methanol exposure. Material and Methods: Methanol intoxication was confirmed analytically in 55 patients included in he d ; hei age a he ime f i ning a 46.7 3.6 ea (9 female and 46 male ). All a ien , together with 41 control subjects, were examined in a prospective longitudinal cohort study. At admission, during hospitalisation, and at regular intervals after discharge during the follow-up, the patients were sampled for serum concentrations of lipid oxidative damage markers 4-hydroxy-trans-2- hexenal (HHE), 4-hydroxynonenal (HNE), malondialdehyde (MDA), and 8-isoprostane, for nucleic acids oxidative damage markers 8-hydroxy-2 -deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8- OHG), 5- (hydroxymethyl) uracil (5-OHMU), for proteins oxidative damage markers ortho-tyrosine (o- Tyr),...