Mutagenní a antimutagenní účinky látek zevního prostředí
Mutagenic and Antimutagenic Effect of Environmental Substances
dissertation thesis (DEFENDED)

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Permanent link
http://hdl.handle.net/20.500.11956/17669Collections
- Kvalifikační práce [2545]
Author
Advisor
Referee
Sedmíková, Markéta
Novotná, Božena
Faculty / Institute
Third Faculty of Medicine
Discipline
-
Department (external)
Information is unavailable
Date of defense
29. 9. 2008
Publisher
Univerzita Karlova, 3. lékařská fakultaLanguage
Czech
Grade
Pass
Keywords
Keywords not foundCílem práce je testování antimutagenních účinků chemicky definovaných přírodních látek: diallylsulfid (DAS), kyselina ellagová (EA), resveratrol (RES) a fenetyl isothiokyanát (PEITC) na experimentálních modelech, které byly ovlivněny vybranými mutageny: aflatoxin B1 (AFB1), 2-amino-3-methylimidazo/4,5,-f/chinolin (IQ) a N-nitroso-N metylurea (MNU). AFB1 a IQ jsou nepřímé mutageny a vyžadují metabolickou aktivaci k tomu, aby se projevil jejich genotoxický efekt. Oproti tomu třetí použitý karcinogen MNU metabolickou aktivaci nevyžaduje. Testováni protektivních látek přírodního původu bylo provedeno na prokaryotním modelu (Amesův test, kmeny Salmonella typhimurium TA98, TA100) a eukaryotním modelu (mikronukleus test a kometový test na myším modelu).
In the present study were used reference mutagens which effects mutagenic activity in prokaryotic and eukaryotic testing systems. Aflatoxin B1 (AFB1) is one of the most thoroughly studied and the well known mycotoxin with carcinogenic activity. 2-Amino-3-methylimidazol[4,5-f] quinoline (IQ) in isolated form has been used as another reference mutagen. These compounds are indirect acting genotoxins, i.e. need metabolic activation to exert genotoxic effect. As a third reference mutagen in the present study there has been chosen N-nitroso-N-methylurea (MNU) which is direct acting carcinogenic N-nitroso compound. The aim of the study was to investigate antimutagenic effects of ellagic acide (EA), resveratrol (RES), diallyl sulfide (DAS) and phenetyl isothiocyanate (PEITC) on the mutagenicity of the mutagens. The Ames test with Salmonella typhimurium TA98 and TA100 strains was used for the evaluation of antimutagenic effect of EA, RES, DAS and PEITC in vitro.