Separace a stanovení farmaceuticky významných sloučenin chemického a přírodního původu s využitím kapilárních elektroforetických metod

Abstract

13. SUMMARY This thesis deals with the results of applications of electrophoretic methods in the separation and determination of pharmaceutical important compounds of synthetic or herbal origin. In the theoretic part, the classification of the capillary electrophoretic methods is presented. Capillary zone electrophoresis, capillary izotachophoresis and micellar elektrokinetic chromatography were used in this work. These electro-migration methods represent extremely potent separation techniques. The most of the theoretic part is devoted to the popular topic of chiral separations. Especially CZE presents a competition to the HPLC methods in the area of chiral separations. Implementation of a chiral selector into a background electrolyte enables more dynamic and faster optimizing conditions used for chiral separation. That means better discrimination and lower price of analysis. In the result section the first three experimental works describe the application of CZE in pharmaceutical analysis to control the content and optical purity of pharmaceutical active compounds. Successful discriminations of racemic mixture of rivastigmine, tamsulosine and sibutramine, their determination in HVLP and optical purity control, show that the developed, optimized and validated CZE methods offer accurate and correct results....