Modulation of body fat content by targeting the lipid metabolism of adipose tissue
dizertační práce (OBHÁJENO)
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http://hdl.handle.net/20.500.11956/95747Identifikátory
SIS: 198868
Kolekce
- Kvalifikační práce [19614]
Autor
Vedoucí práce
Oponent práce
Konvalinka, Jan
Drahota, Zdeněk
Fakulta / součást
Přírodovědecká fakulta
Obor
Biochemie
Katedra / ústav / klinika
Katedra biochemie
Datum obhajoby
19. 1. 2006
Nakladatel
Univerzita Karlova, Přírodovědecká fakultaJazyk
Angličtina
Známka
Prospěl/a
CONCLUSIONS 1. Induction of obesiý resistance in mice by transgenic expression of UCP1 in white fat can be explained by a metabolic switch ínadipocýes, due to depressíon oť celluiar energy charge, in situ activaÍion of AMPK, down- regulation ofadipogenic genes and increase in lipid oxidation. 2. AMPK cascade is involved in the fat depot-specific metabolic responses in various fat depots to starvation. Activation of the cascade occurred in epididymal but not in subcutaneous fat in mice. The activation of AMPK in adipocytes might represent an important mechanism by which body fat stores are regulated and may contributes to regional differences in the metabolic properties ofadipose tissue depots. Dietary EPA and DHA reduce development of obesity induced in mice by high fat diets, in part due to counteracting increase in tissue cellularity, particularly in epididymal íat.Low EPA/DHA ratio potentiates the anti- adipogenic efíect. Dietary EPA and DHA induce a metabolic shift in white adipose tissue by up-regulating genes for mitochondrial proteins, including their regulatory genes PGCIa and NRF-I, and increase B-oxidation while depressing lipogenesis, preferentially in the epididymal fat in the abdomen. 3. 4. J. 21
CONCLUSIONS 1. Induction of obesiý resistance in mice by transgenic expression of UCP1 in white fat can be explained by a metabolic switch ínadipocýes, due to depressíon oť celluiar energy charge, in situ activaÍion of AMPK, down- regulation ofadipogenic genes and increase in lipid oxidation. 2. AMPK cascade is involved in the fat depot-specific metabolic responses in various fat depots to starvation. Activation of the cascade occurred in epididymal but not in subcutaneous fat in mice. The activation of AMPK in adipocytes might represent an important mechanism by which body fat stores are regulated and may contributes to regional differences in the metabolic properties ofadipose tissue depots. Dietary EPA and DHA reduce development of obesity induced in mice by high fat diets, in part due to counteracting increase in tissue cellularity, particularly in epididymal íat.Low EPA/DHA ratio potentiates the anti- adipogenic efíect. Dietary EPA and DHA induce a metabolic shift in white adipose tissue by up-regulating genes for mitochondrial proteins, including their regulatory genes PGCIa and NRF-I, and increase B-oxidation while depressing lipogenesis, preferentially in the epididymal fat in the abdomen. 3. 4. J. 21