Analýza,separace a fyzikálně-chemická charakterizace peptidových hormonů kapilární elektroforézou
Analysis, separation and physico-chemical characterization of peptide hormones by capillary electrophoresis
dizertační práce (OBHÁJENO)
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Trvalý odkaz
http://hdl.handle.net/20.500.11956/95732Identifikátory
SIS: 112780
Kolekce
- Kvalifikační práce [19109]
Autor
Vedoucí práce
Konzultant práce
Kašička, Václav
Oponent práce
Feltl, Ladislav
Coufal, Pavel
Samcová, Eva
Fakulta / součást
Přírodovědecká fakulta
Obor
-
Katedra / ústav / klinika
Katedra analytické chemie
Datum obhajoby
12. 2. 2007
Nakladatel
Univerzita Karlova, Přírodovědecká fakultaJazyk
Čeština
Známka
Prospěl/a
I 11 CoNcr,usroNs The work bas demonstrated that capinary zone elechophoresis and micelrar electokinetic cbromatography are high-perfomrance and high-sensitive methods for qualitative and quantitative anďysis and for physico-chemicď characÚerization ofpeptide hormones. using the strategy for the rational selection of experimental conditions for cE analysis and separations ofpeptides, ionogenic peptides were separated as cations in the classical and isoelectric buffers in the acidic region, pH 2- 2'5, and as anions in amphoteric buffer in weakry arkaline conditions, pH = 8'1. Non-ionogenic peptides were analyzed by miceilar electrokinetic chromatography with micelrar phase consisting of anionic detergent sodium dodecylsulphate in alkaline conditions, pH 8.8. The experimental conditions were selected with the aim to avoid the adsorption of peptides on the capillary wall, which was particularly reached with suppression of dissociation of si'anol gÍoups on the imer capillary wall in the acidic pH region and with electrostatic repursion negatively charged peptides from negatively charged dissociation silanol groups on the inner capillary wall in the alkaline pH region. The pH values of background electrolýes were chosen in the region, where the biggest differences in calculated specific charges of analyzed...