Polyphasic approaches in the taxonomy of green aerophytic algae
Polyfázické přístupy v taxonomii zelených aerofytických řas
dizertační práce (OBHÁJENO)
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Dokument je přístupný pouze ve věcné databázi závěrečných prací v souladu s čl. 18a odst. 5 Studijního a zkušebního řádu Univerzity Karlovy v Praze.
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Trvalý odkaz
http://hdl.handle.net/20.500.11956/94321Identifikátory
SIS: 33071
Kolekce
- Kvalifikační práce [20052]
Autor
Vedoucí práce
Oponent práce
Lukešová, Alena
Rindi, Fabio
Fakulta / součást
Přírodovědecká fakulta
Obor
Botanika
Katedra / ústav / klinika
Katedra botaniky
Datum obhajoby
18. 6. 2008
Nakladatel
Univerzita Karlova, Přírodovědecká fakultaJazyk
Angličtina
Známka
Prospěl/a
Conclusions Traditionaltaxonomyoftendependson single charactersusedfor distin- guishingparticularspecies.ln thisthesis,I haveillustratedapplicationofpoly- phasicapproachesin taxonomyof greenaerophyticalgae.Theseapproachesare basedon thecombinationof variouscharacters(traditionalmorphology,modern molphologicalapproaches,SSU, ITS rDNA andgeneintronsequencing)forthe speciesrecognitionin greenalgae. The morphologicalcharactersaloneareoftennot sufficientfor speciesde- lirnitation,thoughtheycan be usedfor routinespeciesidentificationModern rnorphologicalapproacheslike confocalmicroscopyallow to detectclearmor- phologicaldifferencesarnonginvestigatedcloselyrelatedspecies,evenifat first sightthesemorphologicallyvery uniformalgaehardlydifferatthe morphologi- cal level. A numberof rnorphologicalcharactersdoesnot reflectgeneticrela- tionships.Rathertheyareinfluencedby variousenvironmentalor culturecondi- tions.For example,cell dimensionsin Klebsorntidiuntcanbe distinctivelyinflu- encedby pH, humidity,illLrminationandternperatureconditions.However,with theaid of molecularmarkerswe candetectproperrnorphologicalfeaturesuseful for speciesdifferentiationt. ln .lslerochlorls,correlationtestof geneticdistances and morphologicaldifferencesrevealedusefulnessof particularchloroplast charactersforspeciesdelirnitation....
Conclusions Traditionaltaxonomyoftendependson single charactersusedfor distin- guishingparticularspecies.ln thisthesis,I haveillustratedapplicationofpoly- phasicapproachesin taxonomyof greenaerophyticalgae.Theseapproachesare basedon thecombinationof variouscharacters(traditionalmorphology,modern molphologicalapproaches,SSU, ITS rDNA andgeneintronsequencing)forthe speciesrecognitionin greenalgae. The morphologicalcharactersaloneareoftennot sufficientfor speciesde- lirnitation,thoughtheycan be usedfor routinespeciesidentificationModern rnorphologicalapproacheslike confocalmicroscopyallow to detectclearmor- phologicaldifferencesarnonginvestigatedcloselyrelatedspecies,evenifat first sightthesemorphologicallyvery uniformalgaehardlydifferatthe morphologi- cal level. A numberof rnorphologicalcharactersdoesnot reflectgeneticrela- tionships.Rathertheyareinfluencedby variousenvironmentalor culturecondi- tions.For example,cell dimensionsin Klebsorntidiuntcanbe distinctivelyinflu- encedby pH, humidity,illLrminationandternperatureconditions.However,with theaid of molecularmarkerswe candetectproperrnorphologicalfeaturesuseful for speciesdifferentiationt. ln .lslerochlorls,correlationtestof geneticdistances and morphologicaldifferencesrevealedusefulnessof particularchloroplast charactersforspeciesdelirnitation....