Molecular mechanisms involved in genotoxicity of industrially important monomers (styrene, 1,3-butadiene)
Molecular mechanisms involved in genotoxicity of industrially important monomers (styrene, 1,3-butadiene)
dissertation thesis (DEFENDED)
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http://hdl.handle.net/20.500.11956/93741Identifiers
Study Information System: 77243
Collections
- Kvalifikační práce [19062]
Author
Advisor
Referee
Černá, Marie
Machala, Miroslav
Faculty / Institute
Faculty of Science
Discipline
Molecular and Cell Biology
Department
Department of Genetics and Microbiology
Date of defense
15. 9. 2009
Publisher
Univerzita Karlova, Přírodovědecká fakultaLanguage
English
Grade
Pass
2 Contents ABSTRACT........................................................................................................... 2 1. INTRODUCTION............................................................................................. 4 2. AIMS OF THE PROJECT:.............................................................................. 8 2. MATERIAL AND METHODS:....................................................................... 9 3. RESULTS: ....................................................................................................... 10 4. CONCLUSIONS.............................................................................................. 16 5. REFERENCES................................................................................................ 18 INDEX OF PUBLICATIONS:........................................................................... 21 Publications in extenso, which are the base of PhD. thesis .............................. 21 Publications in extenso, without relationship to the PhD thesis...................... 22 ABSTRACT The evaluation of individual health risk in workers occupationally exposed to industrial xenobiotics requires the use of a large number of parameters reflecting external exposure, internal exposure, biological effects and individual susceptibility....
1 ABSTRACT The evaluation of individual health risk in workers occupationally exposed to industrial xenobiotics requires the use of a large number of parameters reflecting external exposure, internal exposure, biological effects and individual susceptibility. Environmental, occupational and life style-related exposure to mutagenic agents may contribute to cancer risk in humans. To prevent the potentially hazardous effects of such agents it is important to understand their mechanisms of action. Styrene is one of the most important monomer for producing polymers and copolymers in plastics, latex paints and together with 1,3-butadiene (BD) in the manufacture of synthetic rubbers. In this thesis, a large set of parameters, including markers of external and internal exposure and biomarkers of biological effects and susceptibility have been studied in relation to the occupational exposure to both styrene and BD. First part of the present study was focused on evaluating the role of various biomarkers to assess genotoxic effects of above mentioned xenobiotics. Biomarkers reflecting styrene- and BD-induced genotoxicity and mutagenicity: O6 -styrene guanine DNA adducts, haemoglobin adducts, single-strand breaks (SSBs), SSB Endo III sites, chromosomal aberrations (CA), hypoxanthine-guanine phosphoribosyltransferase...