Genome size variation in microalgae and its evolutionary consequences
Variabilita velikosti genomu u mikrořas a její evoluční důsledky
dizertační práce (OBHÁJENO)
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Trvalý odkaz
http://hdl.handle.net/20.500.11956/177704Identifikátory
SIS: 168785
Kolekce
- Kvalifikační práce [19588]
Autor
Vedoucí práce
Oponent práce
Boenigk, Jens
Bureš, Petr
Fakulta / součást
Přírodovědecká fakulta
Obor
Botanika
Katedra / ústav / klinika
Katedra botaniky
Datum obhajoby
27. 9. 2022
Nakladatel
Univerzita Karlova, Přírodovědecká fakultaJazyk
Angličtina
Známka
Prospěl/a
Klíčová slova (česky)
velikost genomu, průtoková cytometrie, variabilita obsahu DNA, zlativky, adaptivní potenciálKlíčová slova (anglicky)
genome size, flow cytometry, DNA content variation, microalgae, golden-brown algae, adaptive potentialEukaryotic organisms exhibit tremendous variability in genome size with no apparent connection to their biological complexity. Although this variation is known to correlate with numerous phenotypic traits, its evolutionary consequences remain widely unknown. This particularly applies to microalgae, where the genome size estimation is often methodologically challenging. Yet, microalgae represent a promising model group to study genome size evolution owing to their lower body complexity, short generation time and large population sizes, the latter two allowing them to quickly respond to environmental challenges. The main aim of this thesis was to enhance our understanding of genome size variation in microalgae and its evolutionary consequences. To do so, together with my co-authors, I summarized the flow cytometry (FCM) protocols used for microalgae and microorganisms possessing small genomes and addressed their limitations resulting mainly from insufficient amounts of biomass, difficulties with nuclei extraction and prominent background noise due to presence of various pigments and secondary metabolites. Further, I provided best practice recommendations that include, among others, analysing young cultures, avoiding long-term cultivation, and testing different isolation buffers and nuclei isolation...
Eukaryotic organisms exhibit tremendous variability in genome size with no apparent connection to their biological complexity. Although this variation is known to correlate with numerous phenotypic traits, its evolutionary consequences remain widely unknown. This particularly applies to microalgae, where the genome size estimation is often methodologically challenging. Yet, microalgae represent a promising model group to study genome size evolution owing to their lower body complexity, short generation time and large population sizes, the latter two allowing them to quickly respond to environmental challenges. The main aim of this thesis was to enhance our understanding of genome size variation in microalgae and its evolutionary consequences. To do so, together with my co-authors, I summarized the flow cytometry (FCM) protocols used for microalgae and microorganisms possessing small genomes and addressed their limitations resulting mainly from insufficient amounts of biomass, difficulties with nuclei extraction and prominent background noise due to presence of various pigments and secondary metabolites. Further, I provided best practice recommendations that include, among others, analysing young cultures, avoiding long-term cultivation, and testing different isolation buffers and nuclei isolation...