Spektroskopické studium role karotenoidů ve fotosyntéze
Spectroscopic Study of the Function of Carotenoids in Photosynthesis
rigorous thesis (RECOGNIZED)
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http://hdl.handle.net/20.500.11956/13682Identifiers
Study Information System: 44901
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- Kvalifikační práce [11196]
Author
Faculty / Institute
Faculty of Mathematics and Physics
Discipline
Biophysics and chemical physics
Department
Department of Chemical Physics and Optics
Date of defense
12. 9. 2007
Publisher
Univerzita Karlova, Matematicko-fyzikální fakultaLanguage
Czech
Grade
Recognized
I Spektroskopicke studium role karotenoidu ve fotosynteze Abstrakt diplornove prace Vypracoval: Martin Loew Vedouci diplomove prace: Prof. RNDr. Jan Hala, DrSc. Pracoviste; Katedra chemicke fyziky a optiky Studium role karotenoidu ve fotosyntezeje rychle se rozvijejici vetev vyzkumu biofyzikalnich a biochemickych zakladu fotosyntezy. Teto problematice se intenzivne venuje i pracoviste nizkoteplotnf laserove laboratofe KChFO MFF UK. Cilem prace byl spektroskopicky popis fotosyntetickych pigmentu izolovanych ze zelenych fas Scenedesmus Ouadricauda a nasledne lokalizace jejich absorpce ve spektru intaktm'ch bunek. Pfed vlastnfm mefenim bylo provedeno nekolik zasadnfch uprav experimentalniho zafizeni v nizkoteplotnf laserove laboratofi. Vyznamneho zlepseni v praci s kapalnym heliem bylo dosazeno instalaci noveho hloubkomeru do ponorneho optickeho kryostatu. Do aparatury pro fluorescencni mefeni byl instalovan novy fotonasobic Hamamatsu, chlazeny Peltierov^m jevem, umoznujici detekci i velmi slabych svetelnych signalu. Absorpcni aparatura byla rozsifena o stabilni zdroj svetla - wolframovou 1000W zarovku napajenou napetim ze stabilizovaneho zdroje. Podafilo se izolovat prakticky vsechny pigrnenty obsazene v fasach: neoxantin, violaxantin, lutein, p-karoten i chlorofyly. Na vzorcich jednotlivych karotenoidu v...
Spectroscopic Study of the Function of Carotenoids in Photosynthesis Abstract of the thesis Author: Martin Loew Supervisor: Prof. RNDr. Jan Hala, DrSc. Department: Department of Chemical Physics and Optics The research of the role of carotenoids in photosynthesis is a fast-growing branch of a biophysical and biochemical research of photosynthesis principles. The Optical Spectroscopy Group of the Department of Chemical Physics and Optics at the Faculty of Mathematics and Physics of Charles University in Prague is fully engaged at this scientific field. The objective of this work was a Spectroscopic characterization of photosynthetic pigments isolated from the green algae Scenedesmus Quadricauda and localization of their absorption within the spectrum of intact cells. Crucial adaptations at the low-temperature setup were carried out prior to the measurements. The new depth recorder was installed inside the optical cryostat, which meant a significant improvement of liquid helium operation. A new Peltier-cooled photomultiplier Hamamatsu was added to the fluorescence measurement setup to enable the detection of very low optical signals. The setup for absorption measurements was equipped with a new stable light source - 1000 W wolfram bulb connected to stabilised power supply. All the pigments contained in green...