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Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimerʼs disease II.
dc.contributor.advisorChlebek, Jakub
dc.creatorČakurdová, Marta
dc.date.accessioned2017-08-08T09:57:25Z
dc.date.available2017-08-08T09:57:25Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/20.500.11956/87428
dc.description.abstractČakurdová, M.: Alkaloidy Papaver rhoeas L. (Papaveraceae) a jejich biologická aktivita vztažená k Alzheimerovej chorobě II. Diplomová práca, Univerzita Karlova v Prahe, Farmaceutická fakulta v Hradci Králové, Katedra farmaceutickej botaniky a ekológie, Hradec Králové, 2017. Cieľom tejto práce je izolácia alkaloidov z frakcie 20-27, ktorá bola získaná zo sumárneho alkaloidného extraktu Papaver rhoeas L. (Papaveraceae). Následne ich identifikácia pomocou štruktúrnej analýzy NMR, GC-MS, optickej otáčavosti a teploty topenia. Pomocou chromatografických metód boli izolované 2 alkaloidy (-)-stylopin a (+)-rhoeadin. U izolovaných látok bola stanovená ich inhibičná aktivita voči acetylcholínesteráze, butyrylcholínesteráze a prolyloligopeptidáze. Získané hodnoty boli stanovené ako IC50: (-)- stylopin (IC50 AChE = 522 ± 67 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM); (+)-rhoeadin (IC50 AChE = 915 ± 64 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM). Žiadna z izolovaných látok nevykazovala lepšiu inhibičnú aktivitu voči AChE a BuChE v porovnaní s galanthaminom (IC50 AChE = 1,71± 0,065 µM, IC50 BuChE =42,30 ± 1,30 µM), huperzinom A (IC50 AChE = 0,033 ± 0,001 µM, IC50 BuChE = >1000 µM) a rivastigminom (IC50 AChE = 0,037 ± 0,001 µM, IC50 BuChE = 0,0033 ± 0,0003 µM). Pri testovaní inhibičnej aktivity voči POP...cs_CZ
dc.description.abstractČakurdová, M.: Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimer's disease II. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017. The aim of this work was focused on isolation of alkaloids from the fraction 1 (joined fractions 20-27), which was obtained from the summary alkaloid extract of Papaver rhoeas L. (Papaveraceae). The alkaloids were identified by structural analysis NMR, GC-MS, optical activity and melting point. Two alkaloids were identified as (-)-stylopine and (+)-rhoeadine. Isolated substances were tested on ability to inhibit the enzymes acetylcholinesterase, butyrylcholinesterase and prolyloligopeptidase. Obtained data were expressed as IC50 values: (-)-stylopine (IC50 AChE = 522 ± 67 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM); (+)-rhoeadine (IC50 AChE = 915 ± 64 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM). None of the isolated substances showed so potent cholinesterase inhibitory activity as the alkaloids galanthamine (IC50 AChE = 1.71± 0.065 µM, IC50 BuChE =42.30 ± 1.30 µM) and huperzine A (IC50 AChE = 0.033 ± 0.001 µM, IC50 BuChE = >1000 µM) or rivastigmine (IC50 AChE = 0.037 ± 0.001 µM, IC50 BuChE = 3.3 ± 0.3 nM)....en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Farmaceutická fakulta v Hradci Královécs_CZ
dc.subjectPapaver rhoeasen_US
dc.subjectPapaveraceaeen_US
dc.subjectAlzheimerʼs diseaseen_US
dc.subjectacetylcholinesteraseen_US
dc.subjectbutyrylcholinesteraseen_US
dc.subjectprolyl oligopeptidaseen_US
dc.subjectPapaver rhoeascs_CZ
dc.subjectPapaveraceaecs_CZ
dc.subjectAlzheimerova chorobacs_CZ
dc.subjectacetylcholinesterasacs_CZ
dc.subjectbutyrylcholinesterasacs_CZ
dc.subjectprolyloligopeptidasacs_CZ
dc.titleAlkaloidy Papaver rhoeas L. (Papaveraceae) a jejich biologická aktivita vztažená k Alzheimerově chorobě II.cs_CZ
dc.typediplomová prácecs_CZ
dcterms.created2017
dcterms.dateAccepted2017-05-31
dc.description.departmentDepartment of Pharmaceutical Botanyen_US
dc.description.departmentKatedra farmaceutické botanikycs_CZ
dc.description.facultyFarmaceutická fakulta v Hradci Královécs_CZ
dc.description.facultyFaculty of Pharmacy in Hradec Královéen_US
dc.identifier.repId168754
dc.title.translatedAlkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimerʼs disease II.en_US
dc.contributor.refereeHošťálková, Anna
thesis.degree.nameMgr.
thesis.degree.levelmagisterskécs_CZ
thesis.degree.disciplinePharmacyen_US
thesis.degree.disciplineFarmaciecs_CZ
thesis.degree.programPharmacyen_US
thesis.degree.programFarmaciecs_CZ
uk.thesis.typediplomová prácecs_CZ
uk.taxonomy.organization-csFarmaceutická fakulta v Hradci Králové::Katedra farmaceutické botanikycs_CZ
uk.taxonomy.organization-enFaculty of Pharmacy in Hradec Králové::Department of Pharmaceutical Botanyen_US
uk.faculty-name.csFarmaceutická fakulta v Hradci Královécs_CZ
uk.faculty-name.enFaculty of Pharmacy in Hradec Královéen_US
uk.faculty-abbr.csFaFcs_CZ
uk.degree-discipline.csFarmaciecs_CZ
uk.degree-discipline.enPharmacyen_US
uk.degree-program.csFarmaciecs_CZ
uk.degree-program.enPharmacyen_US
thesis.grade.csVýborněcs_CZ
thesis.grade.enExcellenten_US
uk.abstract.csČakurdová, M.: Alkaloidy Papaver rhoeas L. (Papaveraceae) a jejich biologická aktivita vztažená k Alzheimerovej chorobě II. Diplomová práca, Univerzita Karlova v Prahe, Farmaceutická fakulta v Hradci Králové, Katedra farmaceutickej botaniky a ekológie, Hradec Králové, 2017. Cieľom tejto práce je izolácia alkaloidov z frakcie 20-27, ktorá bola získaná zo sumárneho alkaloidného extraktu Papaver rhoeas L. (Papaveraceae). Následne ich identifikácia pomocou štruktúrnej analýzy NMR, GC-MS, optickej otáčavosti a teploty topenia. Pomocou chromatografických metód boli izolované 2 alkaloidy (-)-stylopin a (+)-rhoeadin. U izolovaných látok bola stanovená ich inhibičná aktivita voči acetylcholínesteráze, butyrylcholínesteráze a prolyloligopeptidáze. Získané hodnoty boli stanovené ako IC50: (-)- stylopin (IC50 AChE = 522 ± 67 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM); (+)-rhoeadin (IC50 AChE = 915 ± 64 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM). Žiadna z izolovaných látok nevykazovala lepšiu inhibičnú aktivitu voči AChE a BuChE v porovnaní s galanthaminom (IC50 AChE = 1,71± 0,065 µM, IC50 BuChE =42,30 ± 1,30 µM), huperzinom A (IC50 AChE = 0,033 ± 0,001 µM, IC50 BuChE = >1000 µM) a rivastigminom (IC50 AChE = 0,037 ± 0,001 µM, IC50 BuChE = 0,0033 ± 0,0003 µM). Pri testovaní inhibičnej aktivity voči POP...cs_CZ
uk.abstract.enČakurdová, M.: Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimer's disease II. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2017. The aim of this work was focused on isolation of alkaloids from the fraction 1 (joined fractions 20-27), which was obtained from the summary alkaloid extract of Papaver rhoeas L. (Papaveraceae). The alkaloids were identified by structural analysis NMR, GC-MS, optical activity and melting point. Two alkaloids were identified as (-)-stylopine and (+)-rhoeadine. Isolated substances were tested on ability to inhibit the enzymes acetylcholinesterase, butyrylcholinesterase and prolyloligopeptidase. Obtained data were expressed as IC50 values: (-)-stylopine (IC50 AChE = 522 ± 67 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM); (+)-rhoeadine (IC50 AChE = 915 ± 64 µM, IC50 BuChE = >1000 µM, IC50 POP = >790 µM). None of the isolated substances showed so potent cholinesterase inhibitory activity as the alkaloids galanthamine (IC50 AChE = 1.71± 0.065 µM, IC50 BuChE =42.30 ± 1.30 µM) and huperzine A (IC50 AChE = 0.033 ± 0.001 µM, IC50 BuChE = >1000 µM) or rivastigmine (IC50 AChE = 0.037 ± 0.001 µM, IC50 BuChE = 3.3 ± 0.3 nM)....en_US
uk.file-availabilityV
uk.publication.placeHradec Královécs_CZ
uk.grantorUniverzita Karlova, Farmaceutická fakulta v Hradci Králové, Katedra farmaceutické botanikycs_CZ


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