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Characterization of Corynebacterium glutamicum promoters responding to increase of temperature
dc.contributor.advisorNešvera, Jan
dc.creatorZemanová, Martina
dc.date.accessioned2019-05-03T13:57:53Z
dc.date.available2019-05-03T13:57:53Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/20.500.11956/6726
dc.description.abstractMy thesis is focused on characterizationof Corynebacteriumglutamicum promoters responding to increase of temperature.C. glutamicum is the most importantrepresentativeof a gram-positive,non-pathogenic,amino-acidproducing bacteria.Its strainsare industrialproducersof glutamic acid, lysine, threonine, tryptophaneand phenylalanine(Liebl et al., l99l). Amino acids producedby microbial fermentationare used in medicine(as infusionliquids or therapeutics), chemical industry (as detergentsand cosmeticscompounds),food industry (as additives), agriculture and stockbreeding (as growth-regulatoradditives to the animalsfeed)(Hermann,2003). Althoughknowledgeof organizationsof its genesinvolvedin biosynthesis of variousaminoacidsincreasedconsiderablyduringlastyears,still littleis known about molecularsignals controllinggene expression. Since regulationof gene expressionon the level of transcriptionplays crucial role, understandingthe structureand functionof transcriptioninitiationsignals(promoters)is of primary importance.Promoters, which can be easily switahed on and off during fermentationsby externalfactorsmay ensurethe controlledoverexpressionofthe chosengenes.Therefore,the C. glutamicumpromotersrespondingto increaseof temperaturehavebeenisolated. Two differentapproachesfor isolationof the promoterswere employed. First...en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleCharakterizace promotorů Corynebacterium glutamicum regulovaných zvýšenou teplotoucs_CZ
dc.typedizertační prácecs_CZ
dcterms.created2008
dcterms.dateAccepted2008-04-17
dc.description.departmentDepartment of Genetics and Microbiologyen_US
dc.description.departmentKatedra genetiky a mikrobiologiecs_CZ
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId112443
dc.title.translatedCharacterization of Corynebacterium glutamicum promoters responding to increase of temperatureen_US
dc.contributor.refereeLichá, Irena
dc.contributor.refereeFelsberg, Jurgen
dc.identifier.aleph000969076
thesis.degree.namePh.D.
thesis.degree.leveldoktorskécs_CZ
thesis.degree.disciplineMolekulární a buněčná biologiecs_CZ
thesis.degree.disciplineMolecular and Cell Biologyen_US
thesis.degree.programMolekulární a buněčná biologiecs_CZ
thesis.degree.programMolecular and Cell Biologyen_US
uk.thesis.typedizertační prácecs_CZ
uk.taxonomy.organization-csPřírodovědecká fakulta::Katedra genetiky a mikrobiologiecs_CZ
uk.taxonomy.organization-enFaculty of Science::Department of Genetics and Microbiologyen_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.csMolekulární a buněčná biologiecs_CZ
uk.degree-discipline.enMolecular and Cell Biologyen_US
uk.degree-program.csMolekulární a buněčná biologiecs_CZ
uk.degree-program.enMolecular and Cell Biologyen_US
thesis.grade.csProspěl/acs_CZ
thesis.grade.enPassen_US
uk.abstract.enMy thesis is focused on characterizationof Corynebacteriumglutamicum promoters responding to increase of temperature.C. glutamicum is the most importantrepresentativeof a gram-positive,non-pathogenic,amino-acidproducing bacteria.Its strainsare industrialproducersof glutamic acid, lysine, threonine, tryptophaneand phenylalanine(Liebl et al., l99l). Amino acids producedby microbial fermentationare used in medicine(as infusionliquids or therapeutics), chemical industry (as detergentsand cosmeticscompounds),food industry (as additives), agriculture and stockbreeding (as growth-regulatoradditives to the animalsfeed)(Hermann,2003). Althoughknowledgeof organizationsof its genesinvolvedin biosynthesis of variousaminoacidsincreasedconsiderablyduringlastyears,still littleis known about molecularsignals controllinggene expression. Since regulationof gene expressionon the level of transcriptionplays crucial role, understandingthe structureand functionof transcriptioninitiationsignals(promoters)is of primary importance.Promoters, which can be easily switahed on and off during fermentationsby externalfactorsmay ensurethe controlledoverexpressionofthe chosengenes.Therefore,the C. glutamicumpromotersrespondingto increaseof temperaturehavebeenisolated. Two differentapproachesfor isolationof the promoterswere employed. First...en_US
uk.file-availabilityV
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra genetiky a mikrobiologiecs_CZ
thesis.grade.codeP


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