Vlastnosti myších buněk, které byly modifikovány geny pro imunomodulační faktory a endostatin

Abstract

Genesfor two cytokines,one chemokineandgenecoding for oneangiostaticfactor were used in the presentwork for tansfection ofmouse IIPV-16- transformedtumor cells. Main characteristics oftransduced cells werc t€stedrnraTo and,in vivo and,cnrnparedwith theparentaltumor cells. In the first part I useda thymidine-kinase deficient (cTK) cell line designated123IA, which had beenderived from IIPVI6 transformedmouse(C57BL/6) cells MK16. To obain genetically modified cells, 1231Acells were transfectedwith bicistronic plasmids carrying the herpessimplex type 1thymidine kinase(HSV-TK) gen,eandeitherthe genefor the mouseB7-l (CD80) co-stimulatory molecule or the gene for the monocyte-chemoattractantprotsin 1 (MCP-|). For control putposes,a plasmid vector carrying only the HSV-TK genewas used.For comparativepurposesI also used89 cells, previously isolated in our laboratory, which expressthe mousegranulocyte-macrophagecolony stimulation factor (GM-CSF) andHSV-TK gene.All the cell lines testedwere found to be sensitiveto minute amountsof ganciclovir, revealing the production of functional HSV-TK. When inoculatedinto syngeneic mice, cells expressing either GM-CSF or B7-1 were non-oncogenic. Nearly all mice inoculatedwith MCP-l-producingcellsdevelopedtumors.Animalsinjectedwith GM-CSF or B7-l- producing cells were...

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