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Characteristics of HPV16 transformed mice cells, transduced with genes for immunomodulating factors and endostatin
dc.contributor.advisorVonka, Vladimír
dc.creatorLakatošová, Monika
dc.date.accessioned2019-05-03T16:44:10Z
dc.date.available2019-05-03T16:44:10Z
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/20.500.11956/35290
dc.description.abstractGenesfor two cytokines,one chemokineandgenecoding for oneangiostaticfactor were used in the presentwork for tansfection ofmouse IIPV-16- transformedtumor cells. Main characteristics oftransduced cells werc t€stedrnraTo and,in vivo and,cnrnparedwith theparentaltumor cells. In the first part I useda thymidine-kinase deficient (cTK) cell line designated123IA, which had beenderived from IIPVI6 transformedmouse(C57BL/6) cells MK16. To obain genetically modified cells, 1231Acells were transfectedwith bicistronic plasmids carrying the herpessimplex type 1thymidine kinase(HSV-TK) gen,eandeitherthe genefor the mouseB7-l (CD80) co-stimulatory molecule or the gene for the monocyte-chemoattractantprotsin 1 (MCP-|). For control putposes,a plasmid vector carrying only the HSV-TK genewas used.For comparativepurposesI also used89 cells, previously isolated in our laboratory, which expressthe mousegranulocyte-macrophagecolony stimulation factor (GM-CSF) andHSV-TK gene.All the cell lines testedwere found to be sensitiveto minute amountsof ganciclovir, revealing the production of functional HSV-TK. When inoculatedinto syngeneic mice, cells expressing either GM-CSF or B7-1 were non-oncogenic. Nearly all mice inoculatedwith MCP-l-producingcellsdevelopedtumors.Animalsinjectedwith GM-CSF or B7-l- producing cells were...en_US
dc.description.abstractSouhrn Vt6toprAcijsemzkoumalavlastnostimy3lchbun6ktransformovanfchviremHPV16'kfer6 byly genetickymodifikovfny vnesenimgent pro cytokiny' chemokin ajeden angiostaticki gen' Byly testovAnyhlavnlcharakteristikytransdukovanfchbun€kvporovnAnisrodidovkjminddorovfmi buikami in vitro a in viYo VprvnldristijsempouZilabunddnouliniidefrcientnlnabunddnoutymidinkin6zu'ozratenou jakol23IA,kter6bylaodvozenazmy5ich(c57BLl6)ledvinnjchbun€ktransformovarrjchonkogeny E6lETHPV16aaktivovanfmonkogenemH.rasomaEenlchjakoMK16.Builkyl23IAjsem transfekovala bicistronickfmi plazmidy nesoucimi geny pro herpetickou tymidin kin6zu (HSv-Tn, kostimulaini molekulu B7-,1(cD 80) nebochemokin - protein 1 chemoatrahujicimonocyty (MCP-L)' ho kontrolni idely jsem pouZilaplazmid obsahujicljen genpro I/SY-IK' Do testfijsem zahrnulatak€ diive izolovan6 bufiky Bg, kterd byly transdukov6ny geny pro faktor stimulujicl tvorbu kolonii granulocytiamonocytft(GM-cSnaHSV-TK'VSechnyizolovan6liniebylycitliv6kegancikloviru' prokazujice tak piitomnost HSV-TK. po inokulaci syngennimmystm, byly bunky exprimujici GM- CSFa B7-l neonkogenni.Po odkov6nibunEkprodukujicichMCP-I vfvofily t6m6l v5echnymy3i fl(dory. Zvl?rltaimtmizovani bunkami produkujici GM-CSF a B7-l byla chr6ndnapled n6slednou...cs_CZ
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleVlastnosti myších buněk, které byly modifikovány geny pro imunomodulační faktory a endostatincs_CZ
dc.typedizertační prácecs_CZ
dcterms.created2011
dcterms.dateAccepted2011-06-16
dc.description.departmentDepartment of Genetics and Microbiologyen_US
dc.description.departmentKatedra genetiky a mikrobiologiecs_CZ
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId108441
dc.title.translatedCharacteristics of HPV16 transformed mice cells, transduced with genes for immunomodulating factors and endostatinen_US
dc.contributor.refereeReiniš, Milan
dc.contributor.refereeForstová, Jitka
dc.identifier.aleph001394852
thesis.degree.namePh.D.
thesis.degree.leveldoktorskécs_CZ
thesis.degree.discipline-cs_CZ
thesis.degree.discipline-en_US
thesis.degree.programMolekulární a buněčná biologie, genetika a virologiecs_CZ
thesis.degree.programMolecular and Cell Biology, Genetics and Virologyen_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.cs-cs_CZ
uk.degree-discipline.en-en_US
uk.degree-program.csMolekulární a buněčná biologie, genetika a virologiecs_CZ
uk.degree-program.enMolecular and Cell Biology, Genetics and Virologyen_US
thesis.grade.csProspěl/acs_CZ
thesis.grade.enPassen_US
uk.abstract.csSouhrn Vt6toprAcijsemzkoumalavlastnostimy3lchbun6ktransformovanfchviremHPV16'kfer6 byly genetickymodifikovfny vnesenimgent pro cytokiny' chemokin ajeden angiostaticki gen' Byly testovAnyhlavnlcharakteristikytransdukovanfchbun€kvporovnAnisrodidovkjminddorovfmi buikami in vitro a in viYo VprvnldristijsempouZilabunddnouliniidefrcientnlnabunddnoutymidinkin6zu'ozratenou jakol23IA,kter6bylaodvozenazmy5ich(c57BLl6)ledvinnjchbun€ktransformovarrjchonkogeny E6lETHPV16aaktivovanfmonkogenemH.rasomaEenlchjakoMK16.Builkyl23IAjsem transfekovala bicistronickfmi plazmidy nesoucimi geny pro herpetickou tymidin kin6zu (HSv-Tn, kostimulaini molekulu B7-,1(cD 80) nebochemokin - protein 1 chemoatrahujicimonocyty (MCP-L)' ho kontrolni idely jsem pouZilaplazmid obsahujicljen genpro I/SY-IK' Do testfijsem zahrnulatak€ diive izolovan6 bufiky Bg, kterd byly transdukov6ny geny pro faktor stimulujicl tvorbu kolonii granulocytiamonocytft(GM-cSnaHSV-TK'VSechnyizolovan6liniebylycitliv6kegancikloviru' prokazujice tak piitomnost HSV-TK. po inokulaci syngennimmystm, byly bunky exprimujici GM- CSFa B7-l neonkogenni.Po odkov6nibunEkprodukujicichMCP-I vfvofily t6m6l v5echnymy3i fl(dory. Zvl?rltaimtmizovani bunkami produkujici GM-CSF a B7-l byla chr6ndnapled n6slednou...cs_CZ
uk.abstract.enGenesfor two cytokines,one chemokineandgenecoding for oneangiostaticfactor were used in the presentwork for tansfection ofmouse IIPV-16- transformedtumor cells. Main characteristics oftransduced cells werc t€stedrnraTo and,in vivo and,cnrnparedwith theparentaltumor cells. In the first part I useda thymidine-kinase deficient (cTK) cell line designated123IA, which had beenderived from IIPVI6 transformedmouse(C57BL/6) cells MK16. To obain genetically modified cells, 1231Acells were transfectedwith bicistronic plasmids carrying the herpessimplex type 1thymidine kinase(HSV-TK) gen,eandeitherthe genefor the mouseB7-l (CD80) co-stimulatory molecule or the gene for the monocyte-chemoattractantprotsin 1 (MCP-|). For control putposes,a plasmid vector carrying only the HSV-TK genewas used.For comparativepurposesI also used89 cells, previously isolated in our laboratory, which expressthe mousegranulocyte-macrophagecolony stimulation factor (GM-CSF) andHSV-TK gene.All the cell lines testedwere found to be sensitiveto minute amountsof ganciclovir, revealing the production of functional HSV-TK. When inoculatedinto syngeneic mice, cells expressing either GM-CSF or B7-1 were non-oncogenic. Nearly all mice inoculatedwith MCP-l-producingcellsdevelopedtumors.Animalsinjectedwith GM-CSF or B7-l- producing cells were...en_US
uk.file-availabilityV
uk.publication-placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra genetiky a mikrobiologiecs_CZ
thesis.grade.codeP


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