Biological activity of plants metabolites.XVII.Alkaloids of Corydalis yanhusuo W.T.WANG.

Abstract

Univerzita Karlova v Praze Farmaceutická fakulta v Hradci Králové Katedra farmaceutické botaniky a ekologie Kandidát: Gabriella Cipra Konzultant: Doc. RNDr. Lubomir Opletal, CSc. Název diplomové práce: Biologická aktivita obsahových látek rostlin XVII. Alkaloidy Corydalis yanhusuo W.T.Wang. ámci studia ostlin s obsahem alkaloid kte inhibuj aktivitu lidsk e t oc tá n acet lcholineste as a s ov but lcholineste as b l studován taxon Corydalis yanhusuo. K izolaci b lo použito kg suchých hl z imá n ext akt b l p ip aven pe kolac Et t to diplomov p áci b l zp acován výt epek -Et2 p lkaloid tohoto výt epku b l ozd len na baze jejichž chlo id jsou ozpustn a ne ozpustn v chloroformu. Z každ uveden f akce b l dále z skán alkaloid fenolick a nefenolick áce spo vala v d len alkaloid výt epku s obsahem nefenolických alkaloid jejichž chlo id jsou ozpustn v C Cl3. t to sm si b l pomoc sloupcov ch omatog afie na Al2O3 p epa ativn C izolován -ko dalin átka b la identifikována na základ hmotnostn ho spekt a spekte optick otá ivosti a s ovnán m daj s lite á n mi dat i sledován vlivu -ko dalinu na lidskou ChE a BuChE a následn m matematick m výpo tu b la p o -ko dalin zjišt no: IC50 4 ± μ ntioxida n aktivita D test v kázala hodnotu EC50 > μ Klíčová slova: acetylcholinesterasa, butyrylcholinesterasa, alkaloidy,...

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Gabriella Cipra Consultant: Assoc. Prof. RNDr. Lubomir Opletal, CSc. Title of Thesis: Biological activity of plant metabolites XVII. Alkaloids of Corydalis yanhusuo W.T. Wang In the process of screening for plants containing alkaloids potentially inhibiting human erythrocytic AChE and human BuChE, Corydalis yanhusuo was studied. 10.8 kg of dried tuber was percolated with 120 liters of 95% ethanol. From the primary extract, extracts with individual types of alkaloids were prepared. In this diploma thesis only one extract was processed (extract type A-ether, pH 9.7). Alkaloids from this extract were separated into bases which chlorides were either soluble or insoluble in chloroform. From each of the above mentioned fractions phenolic and non- phenolic alkaloids were obtained. Alkaloids were separated from extract of non-phenolic chlorides soluble in chloroform. From this mixture (+)-corydaline (labeled GC-1) was isolated by the use of column chromatography on alumina and preparative TLC on silica gel. This compound was preliminary identified according to data of MS spectra, NMR spectra, and optical rotation and by comparison with literature data. The biological activity of...