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dc.contributor.advisorAnděra, Ladislav
dc.creatorNevařil, Leonard
dc.date.accessioned2017-04-20T10:56:07Z
dc.date.available2017-04-20T10:56:07Z
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/20.500.11956/26185
dc.description.abstractTumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL) is a cytokine of TNF family, which participates in the non-exclusive regulation of survival and proliferation of mainly hematopoietic cells. Shortly after its discovery it also brought significant attention as specific and potent inducer of apoptosis of cancer cells of various origins, and since then it has been investigated as a potential novel anti-tumor therapeutics. Recently, cancer stem cells (CSCs) were suggested to be a distinct subset of tumor cells that could be responsible at least in some tumors for their sustainment, recurrence and drug resistance. These cells in the "hierarchic" model of tumorigenesis thus represent an important and attractive target for efficient tumor therapy. In this study we use several colorectal adenocarcinoma cell lines as an experimental model for the analysis of CSC-prone cultivation conditions on TRAIL-induced apoptosis of these cells. For enrichment of eventual cancer stem cells we cultivated cell lines in a serum-free medium, originally developed for cultivation of neural stem cells, and assessed the expression of putative CSC markers CD133 and ABCG2 by flow cytometry (FACS). Simultaneously, we tested the expression of TRAIL receptors and susceptibility to TRAIL-induced apoptosis in these cells. We...en_US
dc.languageEnglishcs_CZ
dc.language.isoen_US
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleTRAIL-induced Apoptosis in Populations of Colon Cancer Cell Lines under Various Cultivation Conditionsen_US
dc.typediplomová prácecs_CZ
dcterms.created2009
dcterms.dateAccepted2009-09-24
dc.description.departmentDep. of Physiology and Develop. Biology (obsolete)en_US
dc.description.departmentKatedra fyziol. živočichů a vývoj. biol. (zrušena)cs_CZ
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId57082
dc.contributor.refereeNeužil, Jiří
dc.identifier.aleph002090521
thesis.degree.nameMgr.
thesis.degree.levelnavazující magisterskécs_CZ
thesis.degree.disciplineImunologiecs_CZ
thesis.degree.disciplineImmunologyen_US
thesis.degree.programBiologiecs_CZ
thesis.degree.programBiologyen_US
uk.thesis.typediplomová prácecs_CZ
uk.taxonomy.organization-csPřírodovědecká fakulta::Katedra fyziol. živočichů a vývoj. biol. (zrušena)cs_CZ
uk.taxonomy.organization-enFaculty of Science::Dep. of Physiology and Develop. Biology (obsolete)en_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.csImunologiecs_CZ
uk.degree-discipline.enImmunologyen_US
uk.degree-program.csBiologiecs_CZ
uk.degree-program.enBiologyen_US
thesis.grade.csVýborněcs_CZ
thesis.grade.enExcellenten_US
uk.abstract.enTumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL) is a cytokine of TNF family, which participates in the non-exclusive regulation of survival and proliferation of mainly hematopoietic cells. Shortly after its discovery it also brought significant attention as specific and potent inducer of apoptosis of cancer cells of various origins, and since then it has been investigated as a potential novel anti-tumor therapeutics. Recently, cancer stem cells (CSCs) were suggested to be a distinct subset of tumor cells that could be responsible at least in some tumors for their sustainment, recurrence and drug resistance. These cells in the "hierarchic" model of tumorigenesis thus represent an important and attractive target for efficient tumor therapy. In this study we use several colorectal adenocarcinoma cell lines as an experimental model for the analysis of CSC-prone cultivation conditions on TRAIL-induced apoptosis of these cells. For enrichment of eventual cancer stem cells we cultivated cell lines in a serum-free medium, originally developed for cultivation of neural stem cells, and assessed the expression of putative CSC markers CD133 and ABCG2 by flow cytometry (FACS). Simultaneously, we tested the expression of TRAIL receptors and susceptibility to TRAIL-induced apoptosis in these cells. We...en_US
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra fyziol. živočichů a vývoj. biol. (zrušena)cs_CZ
dc.identifier.lisID990020905210106986


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