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Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines
dc.contributor.advisorHejnar, Jiří
dc.creatorKovářová, Denisa
dc.date.accessioned2017-04-21T08:31:58Z
dc.date.available2017-04-21T08:31:58Z
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/20.500.11956/26122
dc.description.abstract4 Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines Abstract v-Src protein tyrosine kinase is the product of the transforming gene transduced by avian Rous sarcoma virus. In contrast to proto-oncogene c-src, v-src lacks the negative-regulatory C-terminal domain and consequently shows a higher level of activity and transforming ability. In addition, v-Src contains point mutations throughout its coding region that probably contribute to the high level of intrinsic kinase activity. Long terminal repeats (LTR) comprise strong promoter-enhancer sequences and ensure efficient expression of the v-src gene. v-Src protein has a strong transforming potential in vitro and induces tumor development and growth in vivo. Moreover, it is implicated in metastatic formation. In several cancer types the elevated c-Src kinase activity caused pleiotropic cellular responses inducing transformation and metastasis. The aim of this diploma work was to reveal the role of v-Src in mediating tumor and metastatic progression in chicken cell lines PR9692 and PR9692-E9. Despite the low propensity of the chicken cells to immortalization, comparatively high immoratlization efficiency was observed in cells from ex vivo tumours growing progressively...en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleZměny onkogenních vlastností nádorových buněk modulací exprese onkogenů. Příklad kuřecích buněčných linií transformovaných genem v-srccs_CZ
dc.typediplomová prácecs_CZ
dcterms.created2009
dcterms.dateAccepted2009-09-21
dc.description.departmentDepartment of Genetics and Microbiologyen_US
dc.description.departmentKatedra genetiky a mikrobiologiecs_CZ
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId60889
dc.title.translatedAlterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell linesen_US
dc.contributor.refereeŠpanielová, Hana
dc.identifier.aleph001223393
thesis.degree.nameMgr.
thesis.degree.levelnavazující magisterskécs_CZ
thesis.degree.disciplineGenetika, molekulární biologie a virologiecs_CZ
thesis.degree.disciplineGenetics, Molecular Biology and Virologyen_US
thesis.degree.programBiologiecs_CZ
thesis.degree.programBiologyen_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.csGenetika, molekulární biologie a virologiecs_CZ
uk.degree-discipline.enGenetics, Molecular Biology and Virologyen_US
uk.degree-program.csBiologiecs_CZ
uk.degree-program.enBiologyen_US
thesis.grade.csVýborněcs_CZ
thesis.grade.enExcellenten_US
uk.abstract.en4 Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines Abstract v-Src protein tyrosine kinase is the product of the transforming gene transduced by avian Rous sarcoma virus. In contrast to proto-oncogene c-src, v-src lacks the negative-regulatory C-terminal domain and consequently shows a higher level of activity and transforming ability. In addition, v-Src contains point mutations throughout its coding region that probably contribute to the high level of intrinsic kinase activity. Long terminal repeats (LTR) comprise strong promoter-enhancer sequences and ensure efficient expression of the v-src gene. v-Src protein has a strong transforming potential in vitro and induces tumor development and growth in vivo. Moreover, it is implicated in metastatic formation. In several cancer types the elevated c-Src kinase activity caused pleiotropic cellular responses inducing transformation and metastasis. The aim of this diploma work was to reveal the role of v-Src in mediating tumor and metastatic progression in chicken cell lines PR9692 and PR9692-E9. Despite the low propensity of the chicken cells to immortalization, comparatively high immoratlization efficiency was observed in cells from ex vivo tumours growing progressively...en_US
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra genetiky a mikrobiologiecs_CZ


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