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The influence of deep hypothermia on the endocrine function of adipose tissue
dc.contributor.advisorHaluzík, Martin
dc.creatorDrápalová, Jana
dc.date.accessioned2021-03-24T10:32:37Z
dc.date.available2021-03-24T10:32:37Z
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/20.500.11956/26115
dc.description.abstractIntroduction and aims: Deep hypothermia is a medical method used in a variety of car- diological operations. Adipose tissue is one of the main endocrine organs and therefore its en- docrine function significantly influences the state of a patient. The aim of this study was to explore the influence of deep hypothermia on the expression of selected proteins in adipose tissue, which has yet to be described in scientific literature. Material and methods: Samples of subcutaneous and visceral fat were taken from 10 pa- tients, who underwent planned surgery for pulmonary hypertension. The samples were taken at the beginning and the end of hypothermia, and at the end of the operation, respctively. Real-time PCR method was used to determine relative gene expression of selected adipocy- tokines (BCL-2, GLUT1, GPX1, HIF1- , IL-6, IRS1, MCP1, MIF, MT3, TNF-) from total fat and isolated adipocytes. Results: Gene expression of GLUT1 (total subcutaneous fat), HIF1- (total subcutaneous and visceral fat) and IL-6 a MCP-1 (in all tissues) at the end of the operation was significantly higher compared to the gene expression at the beginning and end of hypothermia. Gene ex- pression of TNF- in total subcutaneous fat increased significantly at the end of the operation compared to the beginning of hypothermia. Gene...en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Přírodovědecká fakultacs_CZ
dc.titleVliv hluboké hypotermie na endokrinní funkci tukové tkáněcs_CZ
dc.typediplomová prácecs_CZ
dcterms.created2009
dcterms.dateAccepted2009-06-01
dc.description.departmentDep. of Physiology and Develop. Biology (obsolete)en_US
dc.description.departmentKatedra fyziol. živočichů a vývoj. biol. (zrušena)cs_CZ
dc.description.facultyFaculty of Scienceen_US
dc.description.facultyPřírodovědecká fakultacs_CZ
dc.identifier.repId52616
dc.title.translatedThe influence of deep hypothermia on the endocrine function of adipose tissueen_US
dc.contributor.refereeFlachs, Pavel
dc.identifier.aleph002081687
thesis.degree.nameMgr.
thesis.degree.levelnavazující magisterskécs_CZ
thesis.degree.disciplineFyziologie živočichůcs_CZ
thesis.degree.disciplineAnimal Physiologyen_US
thesis.degree.programBiologyen_US
thesis.degree.programBiologiecs_CZ
uk.thesis.typediplomová prácecs_CZ
uk.taxonomy.organization-csPřírodovědecká fakulta::Katedra fyziol. živočichů a vývoj. biol. (zrušena)cs_CZ
uk.taxonomy.organization-enFaculty of Science::Dep. of Physiology and Develop. Biology (obsolete)en_US
uk.faculty-name.csPřírodovědecká fakultacs_CZ
uk.faculty-name.enFaculty of Scienceen_US
uk.faculty-abbr.csPřFcs_CZ
uk.degree-discipline.csFyziologie živočichůcs_CZ
uk.degree-discipline.enAnimal Physiologyen_US
uk.degree-program.csBiologiecs_CZ
uk.degree-program.enBiologyen_US
thesis.grade.csVelmi dobřecs_CZ
thesis.grade.enVery gooden_US
uk.abstract.enIntroduction and aims: Deep hypothermia is a medical method used in a variety of car- diological operations. Adipose tissue is one of the main endocrine organs and therefore its en- docrine function significantly influences the state of a patient. The aim of this study was to explore the influence of deep hypothermia on the expression of selected proteins in adipose tissue, which has yet to be described in scientific literature. Material and methods: Samples of subcutaneous and visceral fat were taken from 10 pa- tients, who underwent planned surgery for pulmonary hypertension. The samples were taken at the beginning and the end of hypothermia, and at the end of the operation, respctively. Real-time PCR method was used to determine relative gene expression of selected adipocy- tokines (BCL-2, GLUT1, GPX1, HIF1- , IL-6, IRS1, MCP1, MIF, MT3, TNF-) from total fat and isolated adipocytes. Results: Gene expression of GLUT1 (total subcutaneous fat), HIF1- (total subcutaneous and visceral fat) and IL-6 a MCP-1 (in all tissues) at the end of the operation was significantly higher compared to the gene expression at the beginning and end of hypothermia. Gene ex- pression of TNF- in total subcutaneous fat increased significantly at the end of the operation compared to the beginning of hypothermia. Gene...en_US
uk.file-availabilityV
uk.grantorUniverzita Karlova, Přírodovědecká fakulta, Katedra fyziol. živočichů a vývoj. biol. (zrušena)cs_CZ
thesis.grade.code2
dc.contributor.consultantVybíral, Stanislav
uk.publication-placePrahacs_CZ
uk.thesis.defenceStatusO


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