| dc.contributor.advisor | Otáhal, Jakub | |
| dc.creator | Hráchová, Anna | |
| dc.date.accessioned | 2017-04-19T14:43:12Z | |
| dc.date.available | 2017-04-19T14:43:12Z | |
| dc.date.issued | 2010 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11956/22208 | |
| dc.description.abstract | Title of work: Model of focal brain ischemic lesion in neonatal rats. Work objectives: The aim of this diploma thesis was to determinate the parameter of the lesion after focal brain ischemia induced by photothrombosis, establish the changes of sensorimotor abilities and investigate the occurrence of post stroke epileptic seizures in this new model in P7 rat pups. Materials and Methods: Intravenous application of bengal rose dye was followed by illumination of concentrated beam of high powered green laser over the sensorimotocortex for 5 minutes at 0,5s on/off cycles. To assess the motor deficit, rotarod walking test and bar holding test were performed in two months after stroke. The animals underwent implantation of EEG cortical and hippocampal electrodes followed by a week of recovery four months after stroke. EEG monitoring for five consecutive days have been done. Then animals were overdosed with urethane and transcardially perfused. The brains were cut into 50μm slice for histological staining. There were another five previous experiments, which were preliminary studies aiming at correct inducing of photothrombotic stroke and also EEG monitoring at all. Every study included a control groups that underwent the same surgical protocol. Present work includes only the last sixth experiment.... | cs_CZ |
| dc.description.abstract | Title of work: Model of focal brain ischemic lesion in neonatal rats. Work objectives: The aim of this diploma thesis was to determinate the parameter of the lesion after focal brain ischemia induced by photothrombosis, establish the changes of sensorimotor abilities and investigate the occurrence of post stroke epileptic seizures in this new model in P7 rat pups. Materials and Methods: Intravenous application of bengal rose dye was followed by illumination of concentrated beam of high powered green laser over the sensorimotocortex for 5 minutes at 0,5s on/off cycles. To assess the motor deficit, rotarod walking test and bar holding test were performed in two months after stroke. The animals underwent implantation of EEG cortical and hippocampal electrodes followed by a week of recovery four months after stroke. EEG monitoring for five consecutive days have been done. Then animals were overdosed with urethane and transcardially perfused. The brains were cut into 50μm slice for histological staining. There were another five previous experiments, which were preliminary studies aiming at correct inducing of photothrombotic stroke and also EEG monitoring at all. Every study included a control groups that underwent the same surgical protocol. Present work includes only the last sixth experiment.... | en_US |
| dc.language | English | cs_CZ |
| dc.language.iso | en_US | |
| dc.publisher | Univerzita Karlova, Fakulta tělesné výchovy a sportu | cs_CZ |
| dc.title | Model of focal ischemic brain lesion in neonatal rat. | en_US |
| dc.type | diplomová práce | cs_CZ |
| dcterms.created | 2010 | |
| dcterms.dateAccepted | 2010-05-12 | |
| dc.description.department | Fyzioterapie | cs_CZ |
| dc.description.faculty | Faculty of Physical Education and Sport | en_US |
| dc.description.faculty | Fakulta tělesné výchovy a sportu | cs_CZ |
| dc.identifier.repId | 85143 | |
| dc.title.translated | Model fokální ischemické léze mozku u neonatálních potkanů | cs_CZ |
| dc.contributor.referee | Brime, Tufikameni | |
| dc.identifier.aleph | 001380363 | |
| thesis.degree.name | Mgr. | |
| thesis.degree.level | navazující magisterské | cs_CZ |
| thesis.degree.discipline | Fyzioterapie | cs_CZ |
| thesis.degree.discipline | Physiotherapy | en_US |
| thesis.degree.program | Specializace ve zdravotnictví | cs_CZ |
| thesis.degree.program | Specializations in Health Service | en_US |
| uk.thesis.type | diplomová práce | cs_CZ |
| uk.taxonomy.organization-cs | Fakulta tělesné výchovy a sportu::Fyzioterapie | cs_CZ |
| uk.faculty-name.cs | Fakulta tělesné výchovy a sportu | cs_CZ |
| uk.faculty-name.en | Faculty of Physical Education and Sport | en_US |
| uk.faculty-abbr.cs | FTVS | cs_CZ |
| uk.degree-discipline.cs | Fyzioterapie | cs_CZ |
| uk.degree-discipline.en | Physiotherapy | en_US |
| uk.degree-program.cs | Specializace ve zdravotnictví | cs_CZ |
| uk.degree-program.en | Specializations in Health Service | en_US |
| thesis.grade.cs | Výborně | cs_CZ |
| thesis.grade.en | Excellent | en_US |
| uk.abstract.cs | Title of work: Model of focal brain ischemic lesion in neonatal rats. Work objectives: The aim of this diploma thesis was to determinate the parameter of the lesion after focal brain ischemia induced by photothrombosis, establish the changes of sensorimotor abilities and investigate the occurrence of post stroke epileptic seizures in this new model in P7 rat pups. Materials and Methods: Intravenous application of bengal rose dye was followed by illumination of concentrated beam of high powered green laser over the sensorimotocortex for 5 minutes at 0,5s on/off cycles. To assess the motor deficit, rotarod walking test and bar holding test were performed in two months after stroke. The animals underwent implantation of EEG cortical and hippocampal electrodes followed by a week of recovery four months after stroke. EEG monitoring for five consecutive days have been done. Then animals were overdosed with urethane and transcardially perfused. The brains were cut into 50μm slice for histological staining. There were another five previous experiments, which were preliminary studies aiming at correct inducing of photothrombotic stroke and also EEG monitoring at all. Every study included a control groups that underwent the same surgical protocol. Present work includes only the last sixth experiment.... | cs_CZ |
| uk.abstract.en | Title of work: Model of focal brain ischemic lesion in neonatal rats. Work objectives: The aim of this diploma thesis was to determinate the parameter of the lesion after focal brain ischemia induced by photothrombosis, establish the changes of sensorimotor abilities and investigate the occurrence of post stroke epileptic seizures in this new model in P7 rat pups. Materials and Methods: Intravenous application of bengal rose dye was followed by illumination of concentrated beam of high powered green laser over the sensorimotocortex for 5 minutes at 0,5s on/off cycles. To assess the motor deficit, rotarod walking test and bar holding test were performed in two months after stroke. The animals underwent implantation of EEG cortical and hippocampal electrodes followed by a week of recovery four months after stroke. EEG monitoring for five consecutive days have been done. Then animals were overdosed with urethane and transcardially perfused. The brains were cut into 50μm slice for histological staining. There were another five previous experiments, which were preliminary studies aiming at correct inducing of photothrombotic stroke and also EEG monitoring at all. Every study included a control groups that underwent the same surgical protocol. Present work includes only the last sixth experiment.... | en_US |
| uk.publication.place | Praha | cs_CZ |
| uk.grantor | Univerzita Karlova, Fakulta tělesné výchovy a sportu, Fyzioterapie | cs_CZ |
| dc.identifier.lisID | 990013803630106986 | |
