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In situ Luminescence Detection of Singlet Oxygen
dc.contributor.advisorDědic, Roman
dc.creatorVyklický, Vojtěch
dc.date.accessioned2017-04-18T12:48:57Z
dc.date.available2017-04-18T12:48:57Z
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/20.500.11956/21121
dc.description.abstractThe thesis summarises already published results of singlet oxygen luminiscence detection in relation to photodynamic therapy treatment. The spectroscopic and photosensitising properties of the photosensitizer TMPyP and their comparison with already published properties of TPPS4 are presented. Also the inuence of phosphatidylcholine on photosensitising properties of TPP and singlet oxygen lifetime was investigated. The thesis is mainly focused on adaptation and optimisation of experimental setup for singlet oxygen luminescence deteciton from the surface of solid samples with time and spectral resolution using optical fibres with luminescence probe. The usability was tested on polymer matrix (PMMA or polystyrene) containing protoporphyrine IX. Finally, the kinetics of singlet oxygen phosphorescence measured from surface of thin layer of 3T3 murine fibroblasts containing TPPS4 or TMPyP are presented. Thereby the feasibility of singlet oxygen detection in situ was demonstrated using this setup.en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Matematicko-fyzikální fakultacs_CZ
dc.titleIn situ luminiscenční detekce singletního kyslíkucs_CZ
dc.typediplomová prácecs_CZ
dcterms.created2009
dcterms.dateAccepted2009-05-25
dc.description.departmentDepartment of Chemical Physics and Opticsen_US
dc.description.departmentKatedra chemické fyziky a optikycs_CZ
dc.description.facultyFaculty of Mathematics and Physicsen_US
dc.description.facultyMatematicko-fyzikální fakultacs_CZ
dc.identifier.repId48616
dc.title.translatedIn situ Luminescence Detection of Singlet Oxygenen_US
dc.contributor.refereeMojzeš, Peter
dc.identifier.aleph001116611
thesis.degree.nameMgr.
thesis.degree.levelnavazující magisterskécs_CZ
thesis.degree.disciplineBiofyzika a chemická fyzikacs_CZ
thesis.degree.disciplineBiophysics and Chemical Physicsen_US
thesis.degree.programFyzikacs_CZ
thesis.degree.programPhysicsen_US
uk.thesis.typediplomová prácecs_CZ
uk.taxonomy.organization-csMatematicko-fyzikální fakulta::Katedra chemické fyziky a optikycs_CZ
uk.taxonomy.organization-enFaculty of Mathematics and Physics::Department of Chemical Physics and Opticsen_US
uk.faculty-name.csMatematicko-fyzikální fakultacs_CZ
uk.faculty-name.enFaculty of Mathematics and Physicsen_US
uk.faculty-abbr.csMFFcs_CZ
uk.degree-discipline.csBiofyzika a chemická fyzikacs_CZ
uk.degree-discipline.enBiophysics and Chemical Physicsen_US
uk.degree-program.csFyzikacs_CZ
uk.degree-program.enPhysicsen_US
thesis.grade.csVýborněcs_CZ
thesis.grade.enExcellenten_US
uk.abstract.enThe thesis summarises already published results of singlet oxygen luminiscence detection in relation to photodynamic therapy treatment. The spectroscopic and photosensitising properties of the photosensitizer TMPyP and their comparison with already published properties of TPPS4 are presented. Also the inuence of phosphatidylcholine on photosensitising properties of TPP and singlet oxygen lifetime was investigated. The thesis is mainly focused on adaptation and optimisation of experimental setup for singlet oxygen luminescence deteciton from the surface of solid samples with time and spectral resolution using optical fibres with luminescence probe. The usability was tested on polymer matrix (PMMA or polystyrene) containing protoporphyrine IX. Finally, the kinetics of singlet oxygen phosphorescence measured from surface of thin layer of 3T3 murine fibroblasts containing TPPS4 or TMPyP are presented. Thereby the feasibility of singlet oxygen detection in situ was demonstrated using this setup.en_US
uk.publication.placePrahacs_CZ
uk.grantorUniverzita Karlova, Matematicko-fyzikální fakulta, Katedra chemické fyziky a optikycs_CZ


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