Účinek MDOC na testikulární tkáň II. Intratestikulární aplikace přípravku s MDOC
The effect of MDOC on testicular tissue II. Intratesticular application of a medicament with MDOC
rigorous thesis (DEFENDED)
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http://hdl.handle.net/20.500.11956/19326Identifiers
Study Information System: 70791
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- Kvalifikační práce [6653]
Author
Advisor
Referee
Herink, Josef
Faculty / Institute
Faculty of Pharmacy in Hradec Králové
Discipline
Pharmacy
Department
Department of Biological and Medical Sciences
Date of defense
20. 3. 2009
Publisher
Univerzita Karlova, Farmaceutická fakulta v Hradci KrálovéLanguage
Czech
Grade
Pass
1 Abstract The aim of my rigorose work was to inspect and assess the effects of time and application of calcium/sodium salt of microdispersed oxidised cellulose (MDOC) to the structural components of testes, especially in the time after an operational localization of one testis into the intraabdominal cavity. We used a model of an experimental unilateral cryptorchidism in the adult male Wistar rats. The surgery was performed under standard conditions using a method introduced in the Department of Biological and Medical Sciences of the Faculty of Pharmacy Charles University. The rats were killed by overdosage of aether aenesthesia and the bilateral testes were removed. The tissues were immediately histologically processed. After the histological modifications we tained the sections with hematoxylin - eosin, green trichrome, Periodic acid Schiff and toluidin blue histological stainings. The evaluation was histological with optical microscope. We compared our results with studies, which are described in the theoretical introduction of my work. In the preparations from the time interval of 7 days after surgery with injection application of 1% solution of calcium/sodium salt of MDOC under the tunica albuginea we randomly measured the diameter of the seminiferous tubules. The diameter of the tubules that...