Účinek MDOC na testikulární tkáň II. Intratestikulární aplikace přípravku s MDOC

Abstract

1 Abstract The aim of my rigorose work was to inspect and assess the effects of time and application of calcium/sodium salt of microdispersed oxidised cellulose (MDOC) to the structural components of testes, especially in the time after an operational localization of one testis into the intraabdominal cavity. We used a model of an experimental unilateral cryptorchidism in the adult male Wistar rats. The surgery was performed under standard conditions using a method introduced in the Department of Biological and Medical Sciences of the Faculty of Pharmacy Charles University. The rats were killed by overdosage of aether aenesthesia and the bilateral testes were removed. The tissues were immediately histologically processed. After the histological modifications we tained the sections with hematoxylin - eosin, green trichrome, Periodic acid Schiff and toluidin blue histological stainings. The evaluation was histological with optical microscope. We compared our results with studies, which are described in the theoretical introduction of my work. In the preparations from the time interval of 7 days after surgery with injection application of 1% solution of calcium/sodium salt of MDOC under the tunica albuginea we randomly measured the diameter of the seminiferous tubules. The diameter of the tubules that...