| dc.contributor.advisor | Jenčo, Jaroslav | |
| dc.creator | Ibrahim, Laureen Reda Youssef | |
| dc.date.accessioned | 2023-07-24T19:51:18Z | |
| dc.date.available | 2023-07-24T19:51:18Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11956/181141 | |
| dc.description.abstract | 39 ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical Botany Candidate: Laureen Reda Youssef Ibrahim Supervisor: RNDr. Jaroslav Jenčo, Ph.D. Title of dissertation thesis: Analysis of selected nutraceuticals by liquid chromatography The extract obtained pellets of Humulus Lupulus L. var Columbus was subjected to a phytochemical study and isolation of its bitter acid constituents. The summary extract was separated into individual fractions by liquid-liquid extraction. After performing HPTLC, GC-MS, and LC-MS analysis, the fraction HL-Hex B was selected and purified further by preparative chromatography. A monocomponent pale yellow solid substance of lupulone was obtained after the final preparative separation. Based on instrumental analysis by reversed phase HPLC with core-shell particles, the analyte was compared to the extract of dietary supplement HopsVital where the presence of bitter acids in the supplement was shown. Key words: nutraceuticals, high performance liquid chromatography, sample preparation, modern stationary phases | en_US |
| dc.language | English | cs_CZ |
| dc.language.iso | en_US | |
| dc.publisher | Univerzita Karlova, Farmaceutická fakulta v Hradci Králové | cs_CZ |
| dc.subject | nutraceuticals | en_US |
| dc.subject | high performance liquid chromatography | en_US |
| dc.subject | sample preparation | en_US |
| dc.subject | modern stationary phases | en_US |
| dc.subject | nutraceutika | cs_CZ |
| dc.subject | vysokoúčinná kapalinová chromatografie | cs_CZ |
| dc.subject | úprava vzorek | cs_CZ |
| dc.subject | moderní stqacionární fáze | cs_CZ |
| dc.title | Analysis of selected nutraceuticals by liquid chromatography | en_US |
| dc.type | diplomová práce | cs_CZ |
| dcterms.created | 2023 | |
| dcterms.dateAccepted | 2023-05-29 | |
| dc.description.department | Katedra farmakognozie a farmaceutické botaniky | cs_CZ |
| dc.description.department | Department of Pharmacognosy and Pharmaceutical Botany | en_US |
| dc.description.faculty | Faculty of Pharmacy in Hradec Králové | en_US |
| dc.description.faculty | Farmaceutická fakulta v Hradci Králové | cs_CZ |
| dc.identifier.repId | 227873 | |
| dc.title.translated | Analýza vybraných nutraceutik pomocí kapalinové chromatografie | cs_CZ |
| dc.contributor.referee | Opletal, Lubomír | |
| thesis.degree.name | Mgr. | |
| thesis.degree.level | magisterské | cs_CZ |
| thesis.degree.discipline | Farmacie | cs_CZ |
| thesis.degree.discipline | Pharmacy | en_US |
| thesis.degree.program | Farmacie | cs_CZ |
| thesis.degree.program | Pharmacy | en_US |
| uk.thesis.type | diplomová práce | cs_CZ |
| uk.taxonomy.organization-cs | Farmaceutická fakulta v Hradci Králové::Katedra farmakognozie a farmaceutické botaniky | cs_CZ |
| uk.taxonomy.organization-en | Faculty of Pharmacy in Hradec Králové::Department of Pharmacognosy and Pharmaceutical Botany | en_US |
| uk.faculty-name.cs | Farmaceutická fakulta v Hradci Králové | cs_CZ |
| uk.faculty-name.en | Faculty of Pharmacy in Hradec Králové | en_US |
| uk.faculty-abbr.cs | FaF | cs_CZ |
| uk.degree-discipline.cs | Farmacie | cs_CZ |
| uk.degree-discipline.en | Pharmacy | en_US |
| uk.degree-program.cs | Farmacie | cs_CZ |
| uk.degree-program.en | Pharmacy | en_US |
| thesis.grade.cs | Velmi dobře | cs_CZ |
| thesis.grade.en | Very good | en_US |
| uk.abstract.en | 39 ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical Botany Candidate: Laureen Reda Youssef Ibrahim Supervisor: RNDr. Jaroslav Jenčo, Ph.D. Title of dissertation thesis: Analysis of selected nutraceuticals by liquid chromatography The extract obtained pellets of Humulus Lupulus L. var Columbus was subjected to a phytochemical study and isolation of its bitter acid constituents. The summary extract was separated into individual fractions by liquid-liquid extraction. After performing HPTLC, GC-MS, and LC-MS analysis, the fraction HL-Hex B was selected and purified further by preparative chromatography. A monocomponent pale yellow solid substance of lupulone was obtained after the final preparative separation. Based on instrumental analysis by reversed phase HPLC with core-shell particles, the analyte was compared to the extract of dietary supplement HopsVital where the presence of bitter acids in the supplement was shown. Key words: nutraceuticals, high performance liquid chromatography, sample preparation, modern stationary phases | en_US |
| uk.file-availability | N | |
| uk.grantor | Univerzita Karlova, Farmaceutická fakulta v Hradci Králové, Katedra farmakognozie a farmaceutické botaniky | cs_CZ |
| thesis.grade.code | 2 | |
| uk.publication-place | Hradec Králové | cs_CZ |
| dc.date.embargoEndDate | 29-05-2026 | |
| uk.embargo.reason | ochrana informací chráněných zvláštním zákonem | cs |
| uk.embargo.reason | protection of information protected by a special law | en |
| uk.thesis.defenceStatus | O | |
