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Bioanalytical methodical approaches for the disposition monitoring of a new potential drug in organism using HPLC-PDA-MS-MS
dc.contributor.advisorNobilis, Milan
dc.creatorCísař, Přemysl
dc.date.accessioned2018-10-19T10:20:38Z
dc.date.available2018-10-19T10:20:38Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/20.500.11956/14216
dc.description.abstract1. Summary The aim of this study consisted in the development and validation of a bioanalytical method for the identification and determination of dimefluron and its metabolites in biomatrices. For the purposes of this study, six expected potential dimefluron metabolites were prepared and identified by NMR and MS. Higher homologue of dimefluron (homodimefluron) was selected and synthesized as an internal standard. The chromatographic columns with various types of the stationary phases were tested. The best results were achieved using a pentafluorophenylpropylsilyl silica gel column rinsed with the mobile phase of acetonitrile- phosphate buffer pH = 3 in a gradient mode. After the validation of the bioanalytical method, this chromatographic system was applied to the disposition study of dimefluron in rats. After an intragastric administration of dimefluron to rats, samples of urine and faeces were collected each 24 hours. The elimination of dimefluron and its phase I and phase II metabolites in urine and faeces was studied. Maximum concentrations of dimefluron derivatives in the excrements were found in the time interval of 24 - 48 hours after the administration. 9-O-Desmethyldimefluron and 3-O-desmethyldimefluron were the principal metabolites found in the rat faeces, while the metabolic products of...en_US
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Farmaceutická fakulta v Hradci Královécs_CZ
dc.titleBioanalytické metodické přístupy při sledování osudu nového potenciálního léčiva v organismu pomocí HPLC-PDA-MS-MScs_CZ
dc.typedizertační prácecs_CZ
dcterms.created2008
dcterms.dateAccepted2008-02-11
dc.description.departmentKatedra farmaceutické chemie a farmaceutické analýzycs_CZ
dc.description.departmentDepartment of Pharmaceutical Chemistry and Pharmaceutical Analysisen_US
dc.description.facultyFaculty of Pharmacy in Hradec Královéen_US
dc.description.facultyFarmaceutická fakulta v Hradci Královécs_CZ
dc.identifier.repId53421
dc.title.translatedBioanalytical methodical approaches for the disposition monitoring of a new potential drug in organism using HPLC-PDA-MS-MSen_US
dc.contributor.refereeChládek, Jaroslav
dc.contributor.refereeWsól, Vladimír
dc.identifier.aleph000942885
thesis.degree.namePh.D.
thesis.degree.leveldoktorskécs_CZ
thesis.degree.disciplineDrug Controlen_US
thesis.degree.disciplineKontrola chemických léčivcs_CZ
thesis.degree.programPharmacyen_US
thesis.degree.programFarmaciecs_CZ
uk.thesis.typedizertační prácecs_CZ
uk.taxonomy.organization-csFarmaceutická fakulta v Hradci Králové::Katedra farmaceutické chemie a farmaceutické analýzycs_CZ
uk.taxonomy.organization-enFaculty of Pharmacy in Hradec Králové::Department of Pharmaceutical Chemistry and Pharmaceutical Analysisen_US
uk.faculty-name.csFarmaceutická fakulta v Hradci Královécs_CZ
uk.faculty-name.enFaculty of Pharmacy in Hradec Královéen_US
uk.faculty-abbr.csFaFcs_CZ
uk.degree-discipline.csKontrola chemických léčivcs_CZ
uk.degree-discipline.enDrug Controlen_US
uk.degree-program.csFarmaciecs_CZ
uk.degree-program.enPharmacyen_US
thesis.grade.csProspěl/acs_CZ
thesis.grade.enPassen_US
uk.abstract.en1. Summary The aim of this study consisted in the development and validation of a bioanalytical method for the identification and determination of dimefluron and its metabolites in biomatrices. For the purposes of this study, six expected potential dimefluron metabolites were prepared and identified by NMR and MS. Higher homologue of dimefluron (homodimefluron) was selected and synthesized as an internal standard. The chromatographic columns with various types of the stationary phases were tested. The best results were achieved using a pentafluorophenylpropylsilyl silica gel column rinsed with the mobile phase of acetonitrile- phosphate buffer pH = 3 in a gradient mode. After the validation of the bioanalytical method, this chromatographic system was applied to the disposition study of dimefluron in rats. After an intragastric administration of dimefluron to rats, samples of urine and faeces were collected each 24 hours. The elimination of dimefluron and its phase I and phase II metabolites in urine and faeces was studied. Maximum concentrations of dimefluron derivatives in the excrements were found in the time interval of 24 - 48 hours after the administration. 9-O-Desmethyldimefluron and 3-O-desmethyldimefluron were the principal metabolites found in the rat faeces, while the metabolic products of...en_US
uk.file-availabilityV
uk.publication.placeHradec Královécs_CZ
uk.grantorUniverzita Karlova, Farmaceutická fakulta v Hradci Králové, Katedra farmaceutické chemie a farmaceutické analýzycs_CZ
thesis.grade.codeP


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