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Spectroscopic Study of the Function of Carotenoids in Photosynthesis
dc.creatorLoew, Martin
dc.date.accessioned2021-05-19T16:32:12Z
dc.date.available2021-05-19T16:32:12Z
dc.date.issued2007
dc.identifier.urihttp://hdl.handle.net/20.500.11956/13682
dc.description.abstractSpectroscopic Study of the Function of Carotenoids in Photosynthesis Abstract of the thesis Author: Martin Loew Supervisor: Prof. RNDr. Jan Hala, DrSc. Department: Department of Chemical Physics and Optics The research of the role of carotenoids in photosynthesis is a fast-growing branch of a biophysical and biochemical research of photosynthesis principles. The Optical Spectroscopy Group of the Department of Chemical Physics and Optics at the Faculty of Mathematics and Physics of Charles University in Prague is fully engaged at this scientific field. The objective of this work was a Spectroscopic characterization of photosynthetic pigments isolated from the green algae Scenedesmus Quadricauda and localization of their absorption within the spectrum of intact cells. Crucial adaptations at the low-temperature setup were carried out prior to the measurements. The new depth recorder was installed inside the optical cryostat, which meant a significant improvement of liquid helium operation. A new Peltier-cooled photomultiplier Hamamatsu was added to the fluorescence measurement setup to enable the detection of very low optical signals. The setup for absorption measurements was equipped with a new stable light source - 1000 W wolfram bulb connected to stabilised power supply. All the pigments contained in green...en_US
dc.description.abstractI Spektroskopicke studium role karotenoidu ve fotosynteze Abstrakt diplornove prace Vypracoval: Martin Loew Vedouci diplomove prace: Prof. RNDr. Jan Hala, DrSc. Pracoviste; Katedra chemicke fyziky a optiky Studium role karotenoidu ve fotosyntezeje rychle se rozvijejici vetev vyzkumu biofyzikalnich a biochemickych zakladu fotosyntezy. Teto problematice se intenzivne venuje i pracoviste nizkoteplotnf laserove laboratofe KChFO MFF UK. Cilem prace byl spektroskopicky popis fotosyntetickych pigmentu izolovanych ze zelenych fas Scenedesmus Ouadricauda a nasledne lokalizace jejich absorpce ve spektru intaktm'ch bunek. Pfed vlastnfm mefenim bylo provedeno nekolik zasadnfch uprav experimentalniho zafizeni v nizkoteplotnf laserove laboratofi. Vyznamneho zlepseni v praci s kapalnym heliem bylo dosazeno instalaci noveho hloubkomeru do ponorneho optickeho kryostatu. Do aparatury pro fluorescencni mefeni byl instalovan novy fotonasobic Hamamatsu, chlazeny Peltierov^m jevem, umoznujici detekci i velmi slabych svetelnych signalu. Absorpcni aparatura byla rozsifena o stabilni zdroj svetla - wolframovou 1000W zarovku napajenou napetim ze stabilizovaneho zdroje. Podafilo se izolovat prakticky vsechny pigrnenty obsazene v fasach: neoxantin, violaxantin, lutein, p-karoten i chlorofyly. Na vzorcich jednotlivych karotenoidu v...cs_CZ
dc.languageČeštinacs_CZ
dc.language.isocs_CZ
dc.publisherUniverzita Karlova, Matematicko-fyzikální fakultacs_CZ
dc.titleSpektroskopické studium role karotenoidů ve fotosyntézecs_CZ
dc.typerigorózní prácecs_CZ
dcterms.created2007
dcterms.dateAccepted2007-09-12
dc.description.departmentDepartment of Chemical Physics and Opticsen_US
dc.description.departmentKatedra chemické fyziky a optikycs_CZ
dc.description.facultyFaculty of Mathematics and Physicsen_US
dc.description.facultyMatematicko-fyzikální fakultacs_CZ
dc.identifier.repId44901
dc.title.translatedSpectroscopic Study of the Function of Carotenoids in Photosynthesisen_US
dc.identifier.aleph000889262
thesis.degree.nameRNDr.
thesis.degree.levelrigorózní řízenícs_CZ
thesis.degree.disciplineBiophysics and chemical physicsen_US
thesis.degree.disciplineBiofyzika a chemická fyzikacs_CZ
thesis.degree.programPhysicsen_US
thesis.degree.programFyzikacs_CZ
uk.thesis.typerigorózní prácecs_CZ
uk.taxonomy.organization-csMatematicko-fyzikální fakulta::Katedra chemické fyziky a optikycs_CZ
uk.taxonomy.organization-enFaculty of Mathematics and Physics::Department of Chemical Physics and Opticsen_US
uk.faculty-name.csMatematicko-fyzikální fakultacs_CZ
uk.faculty-name.enFaculty of Mathematics and Physicsen_US
uk.faculty-abbr.csMFFcs_CZ
uk.degree-discipline.csBiofyzika a chemická fyzikacs_CZ
uk.degree-discipline.enBiophysics and chemical physicsen_US
uk.degree-program.csFyzikacs_CZ
uk.degree-program.enPhysicsen_US
thesis.grade.csUznánocs_CZ
thesis.grade.enRecognizeden_US
uk.abstract.csI Spektroskopicke studium role karotenoidu ve fotosynteze Abstrakt diplornove prace Vypracoval: Martin Loew Vedouci diplomove prace: Prof. RNDr. Jan Hala, DrSc. Pracoviste; Katedra chemicke fyziky a optiky Studium role karotenoidu ve fotosyntezeje rychle se rozvijejici vetev vyzkumu biofyzikalnich a biochemickych zakladu fotosyntezy. Teto problematice se intenzivne venuje i pracoviste nizkoteplotnf laserove laboratofe KChFO MFF UK. Cilem prace byl spektroskopicky popis fotosyntetickych pigmentu izolovanych ze zelenych fas Scenedesmus Ouadricauda a nasledne lokalizace jejich absorpce ve spektru intaktm'ch bunek. Pfed vlastnfm mefenim bylo provedeno nekolik zasadnfch uprav experimentalniho zafizeni v nizkoteplotnf laserove laboratofi. Vyznamneho zlepseni v praci s kapalnym heliem bylo dosazeno instalaci noveho hloubkomeru do ponorneho optickeho kryostatu. Do aparatury pro fluorescencni mefeni byl instalovan novy fotonasobic Hamamatsu, chlazeny Peltierov^m jevem, umoznujici detekci i velmi slabych svetelnych signalu. Absorpcni aparatura byla rozsifena o stabilni zdroj svetla - wolframovou 1000W zarovku napajenou napetim ze stabilizovaneho zdroje. Podafilo se izolovat prakticky vsechny pigrnenty obsazene v fasach: neoxantin, violaxantin, lutein, p-karoten i chlorofyly. Na vzorcich jednotlivych karotenoidu v...cs_CZ
uk.abstract.enSpectroscopic Study of the Function of Carotenoids in Photosynthesis Abstract of the thesis Author: Martin Loew Supervisor: Prof. RNDr. Jan Hala, DrSc. Department: Department of Chemical Physics and Optics The research of the role of carotenoids in photosynthesis is a fast-growing branch of a biophysical and biochemical research of photosynthesis principles. The Optical Spectroscopy Group of the Department of Chemical Physics and Optics at the Faculty of Mathematics and Physics of Charles University in Prague is fully engaged at this scientific field. The objective of this work was a Spectroscopic characterization of photosynthetic pigments isolated from the green algae Scenedesmus Quadricauda and localization of their absorption within the spectrum of intact cells. Crucial adaptations at the low-temperature setup were carried out prior to the measurements. The new depth recorder was installed inside the optical cryostat, which meant a significant improvement of liquid helium operation. A new Peltier-cooled photomultiplier Hamamatsu was added to the fluorescence measurement setup to enable the detection of very low optical signals. The setup for absorption measurements was equipped with a new stable light source - 1000 W wolfram bulb connected to stabilised power supply. All the pigments contained in green...en_US
uk.file-availabilityV
uk.grantorUniverzita Karlova, Matematicko-fyzikální fakulta, Katedra chemické fyziky a optikycs_CZ
thesis.grade.codeU
uk.publication-placePrahacs_CZ
uk.thesis.defenceStatusU
dc.identifier.lisID990008892620106986


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